1. Antiherpetic activity of a sulfated polysaccharide from Agaricus brasiliensis mycelia.
- Author
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Cardozo FT, Camelini CM, Mascarello A, Rossi MJ, Nunes RJ, Barardi CR, de Mendonça MM, and Simões CM
- Subjects
- Agaricus growth & development, Animals, Antiviral Agents chemistry, Antiviral Agents isolation & purification, Chlorocebus aethiops, Herpes Simplex virology, Herpesvirus 1, Human physiology, Herpesvirus 2, Human physiology, Humans, Mycelium chemistry, Mycelium growth & development, Polysaccharides chemistry, Polysaccharides isolation & purification, Vero Cells, Agaricus chemistry, Antiviral Agents pharmacology, Herpesvirus 1, Human drug effects, Herpesvirus 2, Human drug effects, Polysaccharides pharmacology
- Abstract
Sulfated polysaccharides are good candidates for drug discovery in the treatment of herpetic infections. Agaricus brasiliensis (syn A. subrufescens, A. blazei) is a Basidiomycete fungus native to the Atlantic forest region of Southeastern Brazil. Herein we report the chemical modification of a polysaccharide extracted from A. brasiliensis mycelia to obtain its sulfated derivative (MI-S), which presented a promising inhibitory activity against HSV-1 [KOS and 29R (acyclovir-resistant) strains] and HSV-2 strain 333, with selectivity indices (SI = CC50/IC50) higher than 439, 208, and 562, respectively. The mechanisms underlying this inhibitory activity were scrutinized by plaque assay with different methodological strategies. MI-S had no virucidal effects, but inhibited HSV-1 and HSV-2 attachment, penetration, and cell-to-cell spread, as well as reducing the expression of HSV-1 ICP27, UL42, gB, and gD proteins. MI-S also presented synergistic antiviral effect with acyclovir. These results suggest that MI-S presents multiple modes of anti-HSV action., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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