1. Revisiting the taxonomy of the genus Elizabethkingia using whole-genome sequencing, optical mapping, and MALDI-TOF, along with proposal of three novel Elizabethkingia species: Elizabethkingia bruuniana sp. nov., Elizabethkingia ursingii sp. nov., and Elizabethkingia occulta sp. nov
- Author
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Brian Emery, Alexis Criscuolo, Dominique Clermont, James Graziano, Melissa Bell, Sylvain Brisse, Chantal Bizet, Phalasy Juieng, Ben W. Humrighouse, Christopher A. Gulvik, John R. McQuiston, Jarrett Gartin, Ainsley C. Nicholson, Vladimir N. Loparev, and Anne M. Whitney
- Subjects
DNA, Bacterial ,0301 basic medicine ,food.ingredient ,Elizabethkingia ,030106 microbiology ,Elizabethkingia miricola ,Zoology ,medicine.disease_cause ,Microbiology ,Article ,Evolution, Molecular ,03 medical and health sciences ,food ,medicine ,DNA Barcoding, Taxonomic ,Elizabethkingia meningoseptica ,Molecular Biology ,Phylogeny ,Whole genome sequencing ,Genetics ,Whole Genome Sequencing ,biology ,Computational Biology ,Nucleic Acid Hybridization ,Genomics ,General Medicine ,biology.organism_classification ,Bacterial Typing Techniques ,Type species ,Phenotype ,030104 developmental biology ,Elizabethkingia endophytica ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Elizabethkingia anophelis ,Taxonomy (biology) ,Flavobacteriaceae ,Genome, Bacterial - Abstract
The genus Elizabethkingia is genetically heterogeneous, and the phenotypic similarities between recognized species pose challenges in correct identification of clinically derived isolates. In addition to the type species Elizabethkingia meningoseptica, and more recently proposed Elizabethkingia miricola, Elizabethkingia anophelis and Elizabethkingia endophytica, four genomospecies have long been recognized. By comparing historic DNA–DNA hybridization results with whole genome sequences, optical maps, and MALDI-TOF mass spectra on a large and diverse set of strains, we propose a comprehensive taxonomic revision of this genus. Genomospecies 1 and 2 contain the type strains E. anophelis and E. miricola, respectively. Genomospecies 3 and 4 are herein proposed as novel species named as Eliza-bethkingia bruuniana sp. nov. (type strain, G0146T = DSM 2975T = CCUG 69503T = CIP 111191T) and Elizabethkingia ursingii sp. nov. (type strain, G4122T = DSM 2974T = CCUG 69496T = CIP 111192T), respectively. Finally, the new species Elizabethkingia occulta sp. nov. (type strain G4070T = DSM 2976T = CCUG 69505T = CIP 111193T), is proposed.
- Published
- 2017
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