1. Characterization and Transferability of Microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)
- Author
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Bronwyn M. Ayre, David G. Roberts, Richard J.N. Allcock, Janet M. Anthony, and Siegfried L. Krauss
- Subjects
0106 biological sciences ,Genetics ,education.field_of_study ,biology ,Anigozanthos manglesii ,Population ,Locus (genetics) ,Plant Science ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Genetic marker ,Evolutionary biology ,Genetic structure ,Microsatellite ,Anigozanthos ,education ,Ecology, Evolution, Behavior and Systematics ,Haemodoraceae ,010606 plant biology & botany - Abstract
Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species. Methods and Results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability. Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.
- Published
- 2017
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