1. Cloning of the citrate permease gene of Lactococcus lactis subsp. lactis biovar diacetylactis and expression in Escherichia coli
- Author
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A. P. De Ruiz Holgado, Daniela Gardiol, Fernando Sesma, and Diego de Mendoza
- Subjects
Biovar ,Restriction Mapping ,Biological Transport, Active ,Gene Expression ,Organic Anion Transporters ,lac operon ,Molecular cloning ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Citric Acid ,Plasmid ,Bacterial Proteins ,Escherichia ,Escherichia coli ,medicine ,Citrates ,Cloning, Molecular ,Ecology ,biology ,Permease ,Lactococcus lactis ,Membrane Transport Proteins ,biology.organism_classification ,Molecular biology ,Biochemistry ,Genes, Bacterial ,bacteria ,Plasmids ,Research Article ,Food Science ,Biotechnology - Abstract
The citrate plasmid (Cit+ plasmid) from Lactococcus lactis subsp. lactis biovar diacetylactis was cloned into the EcoRI site of plasmid pUC18. This recombinant plasmid enabled Escherichia coli K-12 to transport and utilize citrate as a source of energy, indicating expression of the citrate permease from L. lactis biovar diacetylactis. The citrate permease was under the control of the lac promoter of pUC18. Genetic expression of the Cit+ plasmid in maxicells revealed that the plasmid encoded two polypeptides of 47 and 32 kilodaltons, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
- Published
- 1990
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