1. Statistical Assessment of Variability of Terminal Restriction Fragment Length Polymorphism Analysis Applied to Complex Microbial Communities
- Author
-
Emmanuelle Rohrbach, Pierre Rossi, François Gillet, Christof Holliger, Nouhou Diaby, Laboratory of Environmental Biotechnology, Ecole Polytechnique Fédérale de Lausanne ( EPFL ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Laboratoire des systèmes écologiques ( ECOS ), Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), and Laboratoire des systèmes écologiques (ECOS)
- Subjects
DNA, Bacterial ,Ribosomal-Rna Genes ,Ribosomal rna gene ,Computational biology ,Biology ,Bacterial Physiological Phenomena ,Applied Microbiology and Biotechnology ,Restriction fragment ,Bacterial Communities ,03 medical and health sciences ,Bias ,[ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology ,T-RFLP ,RNA, Ribosomal, 16S ,Genetic variation ,Methods ,Amplified Fragment Length Polymorphism Analysis ,Patterns ,Profiles ,Ecosystem ,RNA RIBOSOMAL 16S ,030304 developmental biology ,Genetics ,[ SDE.BE ] Environmental Sciences/Biodiversity and Ecology ,Diversity ,0303 health sciences ,Bacteria ,Ecology ,030306 microbiology ,Genetic Variation ,Primers ,Terminal restriction fragment length polymorphism ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,biology.protein ,Polymorphism analysis ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Restriction fragment length polymorphism ,Polymorphism, Restriction Fragment Length ,Food Science ,Biotechnology - Abstract
The variability of terminal restriction fragment polymorphism analysis applied to complex microbial communities was assessed statistically. Recent technological improvements were implemented in the successive steps of the procedure, resulting in a standardized procedure which provided a high level of reproducibility.
- Published
- 2009
- Full Text
- View/download PDF