1. Assessment of dust sampling methods for the study of cultivable-microorganism exposure in stables
- Author
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Normand, A.C., Vacheyrou, M., Sudre, B., Heederik, D., Piarroux, R., Risk Assessment of Toxic and Immunomodulatory Agents, Dep IRAS, Risk Assessment of Toxic and Immunomodulatory Agents, Dep IRAS, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Risk Assessment Sciences Institute, Utrecht University [Utrecht], laboratoire de parasitologie/mycologie, Hôpital de la Timone [CHU - APHM] (TIMONE), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), and Hôpital de la Timone [CHU - APHM] ( TIMONE )
- Subjects
Veterinary medicine ,MESH: Asthma ,Microorganism ,Microbial diversity ,MESH : Housing, Animal ,Air Microbiology ,MESH : Animals, Domestic ,010501 environmental sciences ,01 natural sciences ,Applied Microbiology and Biotechnology ,MESH: Dust ,MESH: Air Microbiology ,MESH: Risk Factors ,Risk Factors ,MESH: Endotoxins ,MESH : Dust ,Methods ,MESH: Animals ,MESH : Environmental Monitoring ,MESH : Allergens ,2. Zero hunger ,0303 health sciences ,Ecology ,Environmental exposure ,MESH : Risk Factors ,Housing, Animal ,Environmental chemistry ,Animals, Domestic ,Epidemiological Monitoring ,France ,dust ,MESH: Environmental Monitoring ,Biotechnology ,MESH : Endotoxins ,Pairwise correlation ,Air sampling ,Dust sample ,MESH: Allergens ,MESH: Environmental Exposure ,Coronacrisis-Taverne ,MESH : Air Microbiology ,[ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,MESH : Asthma ,03 medical and health sciences ,Animals ,MESH: Animals, Domestic ,MESH : France ,0105 earth and related environmental sciences ,Exposure assessment ,environmental monitoring ,030306 microbiology ,Animal ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Environmental Exposure ,Allergens ,MESH: Housing, Animal ,Asthma ,respiratory tract diseases ,MESH: France ,Endotoxins ,Dust fall ,13. Climate action ,Housing ,Environmental science ,MESH : Animals ,MESH : Environmental Exposure ,Food Science - Abstract
Studies have shown a link between living on a farm, exposure to microbial components (e.g., endotoxins or β- d -glucans), and a lower risk for allergic diseases and asthma. Due to the lack of validated sampling methods, studies of asthma and atopy have not relied on exposure assessment based on culture techniques. Our objective was therefore to compare several dust sampling methods for the detection of cultivable-microorganism exposure in stables. Sixteen French farms were sampled using four different methods: (i) active air sampling using a pump, (ii) passive dust sampling with a plastic box, (iii) dust sampling with an electrostatic dust fall collector (wipe), and (iv) dust sampling using a spatula to collect dust already settled on a windowsill. The results showed that collection of settled dust samples with either plastic boxes or wipes was reproducible (pairwise correlations, 0.72 and 0.73, respectively) and resulted in highly correlated results (pairwise correlation between the two methods, 0.82). We also found that settled dust samples collected with a plastic box correctly reflected the composition of the samples collected in the air of the stable when there was no farmer activity. A loss of microbial diversity was observed when dust was kept for 3 months at room temperature. We therefore conclude that measurement of viable microorganisms within a reasonable time frame gives an accurate representation of the microbial composition of stable air.
- Published
- 2009