1. Evidence for horizontal transfer of 1-aminocyclopropane-1-carboxylate deaminase genes.
- Author
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Hontzeas N, Richardson AO, Belimov A, Safronova V, Abu-Omar MM, and Glick BR
- Subjects
- DNA, Bacterial analysis, DNA, Bacterial genetics, DNA, Ribosomal analysis, Gram-Negative Bacteria genetics, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S genetics, Rhodococcus genetics, Carbon-Carbon Lyases genetics, Gene Transfer, Horizontal, Gram-Negative Bacteria enzymology, Rhodococcus enzymology
- Abstract
PCR was used to rapidly identify and isolate 1-aminocyclopropane-1-carboxylate (ACC) deaminase genes from bacteria. The Shimodaira-Hasegawa test was used to assess whether phylogenetically anomalous gene placements suggestive of horizontal gene transfer (HGT) were significantly favored over vertical transmission. The best maximum likelihood (ML) ACC deaminase tree was significantly more likely than four alternative ML trees, suggesting HGT.
- Published
- 2005
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