1. Enzymatic synthesizing of phytosterol oleic esters.
- Author
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Pan X, Chen B, Wang J, Zhang X, Zhul B, and Tan T
- Subjects
- Enzyme Stability, Enzymes, Immobilized metabolism, Esterification, Esters chemistry, Esters metabolism, Phytosterols chemistry, Phytosterols metabolism, Silica Gel, Solvents, Substrate Specificity, Temperature, Time Factors, Candida enzymology, Esters chemical synthesis, Lipase metabolism, Oleic Acids chemical synthesis, Phytosterols chemical synthesis
- Abstract
A method of synthesizing the phytosterol esters from oleic acid and sterols was studied, using immobilized lipase Candida sp. 99-125 as catalyst. Molar ratio (oleic acid/phytosterols), temperature, reaction period, organic solvents, catalyst, and silica-gel drier were optimized, and the result showed that 93.4% of the sterols had been esterified under the optimal synthetic condition: the molar ratio of oleic acid/phytosterol is 1:1 in 10 mL iso-octane, immobilized lipase (w, 140% of the sterols), incubated in an orbital shaker (200 rpm) at a temperature of 45 °C for 24 h. The immobilized lipase could be reused for at least 13 times with limited loss of esterification activity. The conversion still maintained up to 86.6%. Hence, this developed process for synthesizing phytosterol esters could be considered as simple and low-energy consumption compared to existing chemical processes.
- Published
- 2012
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