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Immunogenic properties of synthetic peptides corresponding to regions 122–133, 136–147, 154–164, and 314–328 of the heavy chain (HA1) of A/Aichi/2/68 virus hemagglutinin were studied. Peptides 122–133 and 136–147 together form a nearly complete antigenic determinant A, peptide 154–164 is a part of determinant B, and peptide 314–328 corresponds to the C-terminal HA1 fragment. In a model influenza A/Aichi/2/68 infection in CBA mice, a protective effect of conjugates of BSA with peptides 136–147 and 314–328 was shown. Immunization of animals with conjugates BSA-(136–147) and BSA-(314–328) in combination with interferon inducers (larifan and ridostin) and a plant immunomodulator (immunomax) intensified the protection of mice against the influenza infection.

Roller culturing of MDCK and Vero cells in an experimental nutrient medium based on soy flour hydrolysate, plant material obtained using the bromelain plant enzyme, was studied. The medium supplemented with 2 or 3% fetal calf serum (FCS) had a strong growth-stimulating effect on Vero and MDCK and cells, respectively, and did not alter the cell morphology. A/Solomon Islands/03/06 (H1N1) and B/Malaysia/2506/04 influenza vaccine viruses were grown on MDCK and Vero cell cultures obtained as a result of culturing in rollers on media containing soy flour hydrolysate and FCS (2 or 3%, respectively). The titer of the viruses was high in the presence of either trypsin (2 μg/ml) or bromelain (20 μg/ml).