1. Biotransformation of benzene and toluene to catechols by phenol hydroxylase from Arthrobacter sp. W1.
- Author
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Ma, Fang, Shi, Sheng-Nan, Sun, Tie-Heng, Li, Ang, Zhou, Ji-Ti, and Qu, Yuan-Yuan
- Subjects
CATECHOL ,BIOTRANSFORMATION (Metabolism) ,BENZENE ,TOLUENE ,HYDROXYLATION - Abstract
Phenol hydroxylase gene engineered microorganism (PH) was used to synthesize catechols from benzene and toluene by successive hydroxylation reaction. HPLC-MS and H NMR analysis proved that the products of biotransformation were the corresponding catechols via the intermediate production of phenols. It was indicated that the main products of toluene oxidation were o-cresol and p-cresol. 3-Methylcatechol was the predominant product for m-cresol biotransformation. Formation rate of catechol (25 μM/min/g cell dry weight) was 1.43-fold higher than that of methylcatechols. It was suggested that phenol hydroxylase could be successfully used to transform both benzene and toluene to catechols by successive hydroxylation. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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