1. Key sites insight on the stereoselectivity of four mined aldo-keto reductases toward α-keto esters and halogen-substituted acetophenones.
- Author
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Zhang, Wenhe, Zhu, Tianhui, Li, Hengyu, Qin, Fengyu, Zhang, Feiting, Zhang, Rui, Jia, Xian, Qin, Bin, and You, Song
- Subjects
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ALDO-keto reductases , *STEREOSELECTIVE reactions , *ESTERS , *ASYMMETRIC synthesis , *ACETOPHENONE derivatives , *ESTER derivatives - Abstract
Biocatalytic reduction catalyzed by aldo-keto reductases (AKRs) is a valuable approach for asymmetric synthesis of chiral alcohols. In this study, four novel aldo-keto reductases with significant activity and stereoselectivity toward a variety of α-keto esters and halogen-substituted acetophenones were identified by genome mining. Through analysis of the crystal structure and multiple-sequence alignment of the starting AKR YvgN from Bacillus subtilis, residues F25 and W113 were proposed as the key positions that might control the stereoselectivity of YvgN. F25S and F25S/W113F variants of YvgN were able to improve its activity and stereoselectivity toward some α-keto ester compounds and halogen-substituted acetophenone derivatives. In addition, similar enhancement of catalytic activity and stereoselectivity was also found in the other three AKRs with corresponding mutations of starting YvgN. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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