1. Squeezed light induced two-photon absorption fluorescence of fluorescein biomarkers
- Author
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Charles Altuzarra, Anton Classen, Tian Li, G. S. Agarwal, and Fu Li
- Subjects
010302 applied physics ,Dye laser ,Materials science ,Physics and Astronomy (miscellaneous) ,FOS: Physical sciences ,Physics::Optics ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Laser ,01 natural sciences ,Two-photon absorption ,Fluorescence ,Molecular physics ,law.invention ,chemistry.chemical_compound ,chemistry ,law ,0103 physical sciences ,Fluorescein ,0210 nano-technology ,Absorption (electromagnetic radiation) ,Preclinical imaging ,Optics (physics.optics) ,Physics - Optics ,Squeezed coherent state - Abstract
Two-photon absorption (TPA) fluorescence of biomarkers has been decisive in advancing the fields of biosensing and deep-tissue in vivo imaging of live specimens. However, due to the extremely small TPA cross section and the quadratic dependence on the input photon flux, extremely high peak-intensity pulsed lasers are imperative, which can result in significant photo- and thermal-damage. Previous works on entangled TPA (ETPA) with spontaneous parametric down-conversion (SPDC) light sources found a linear dependence on the input photon-pair flux, but are limited by low optical powers, along with a very broad spectrum. We report that by using a high-flux squeezed light source for TPA, a fluorescence enhancement of 47 is achieved in fluorescein biomarkers as compared to classical TPA. Moreover, a polynomial behavior of the TPA rate is observed in the DCM laser dye., 5 pages, 4 figures
- Published
- 2020
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