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Your search keyword '"Jun‐Yan Li"' showing total 2 results
Start Over Author "Jun‐Yan Li" Journal applied physics letters
2 results on '"Jun‐Yan Li"'

1. Detection of specific DNA using a microfluidic device featuring tethered poly(N-isopropylacrylamide) on a silicon substrate

Author

Jun-Yan Li and Jem-Kun Chen

Subjects
Chromatography, Physics and Astronomy (miscellaneous), Microfluidics, Substrate (chemistry), Nanotechnology, law.invention, Electrophoresis, genomic DNA, chemistry.chemical_compound, chemistry, law, Agarose gel electrophoresis, Poly(N-isopropylacrylamide), Polymerase chain reaction, DNA
Abstract

In this study, we grafted thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) onto a Si substrate as the medium in a microfluidic device to detect specific DNA molecules [human genomic DNA (hgDNA528), 528 bp] at extremely low concentrations (down to 2 ng/μl). After using the polymerase chain reaction to amplify the released human gDNA signal from the tethered PNIPAAm on the substrate, the amplified human gDNA molecules were characterized through agarose gel electrophoresis. The tethered PNIPAAm in the fluid device allowed the precise detection of the human gDNA.

Published
2010
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2. Detection of specific DNA using a microfluidic device featuring tethered poly(N-isopropylacrylamide) on a silicon substrate.

Author

Jem-Kun Chen and Jun-Yan Li

Subjects
*DNA, *MOLECULES, *MICROFLUIDIC devices, *MICROFLUIDICS, *SILICON, *POLYMERASE chain reaction
Abstract

In this study, we grafted thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) onto a Si substrate as the medium in a microfluidic device to detect specific DNA molecules [human genomic DNA (hgDNA528), 528 bp] at extremely low concentrations (down to 2 ng/μl). After using the polymerase chain reaction to amplify the released human gDNA signal from the tethered PNIPAAm on the substrate, the amplified human gDNA molecules were characterized through agarose gel electrophoresis. The tethered PNIPAAm in the fluid device allowed the precise detection of the human gDNA. [ABSTRACT FROM AUTHOR]

Published
2010
Full Text
View/download PDF

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