1. Label-free electrochemical detection of botulinum neurotoxin type E based on its enzymatic activity using interdigitated electrodes
- Author
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Daehee Kim, Yu Mi Shin, Sang Hwa Hyun, Wan Soo Yun, Ji-Joon Song, Soohyun Kim, Aeyeon Kang, and Dae Keun Park
- Subjects
chemistry.chemical_classification ,Chromatography ,Physics and Astronomy (miscellaneous) ,Toxin ,Chemistry ,010401 analytical chemistry ,Peptide ,02 engineering and technology ,021001 nanoscience & nanotechnology ,medicine.disease_cause ,Cleavage (embryo) ,Electrochemistry ,01 natural sciences ,Redox ,0104 chemical sciences ,Microelectrode ,Biochemistry ,Colloidal gold ,medicine ,Cyclic voltammetry ,0210 nano-technology - Abstract
We report a simple label-free electrochemical method of detecting low concentrations of botulinum neurotoxin type E light chain (BoNT/E LC) based on its peptide cleavage activity. Dual-mode cyclic voltammetry was employed to observe changes in the redox signal of ferri-/ferro-cyanide on interdigitated microelectrodes, whose surfaces were covered by peptides designed from synaptosomal-associated protein 25 to be cleaved by BoNT/E LC. With the introduction of BoNT/E LC, the redox signal showed a time-dependent increase due to cleavage of the immobilized peptide molecules. In addition to the increased redox signal intensity, its time-dependence can be considered as a strong evidence of BoNT/E sensing, since the time-dependent increase can only result from the enzymatic activity of BoNT/E LC. Using this method, BoNT/E LC, at concentrations as low as 5 pg/ml, was readily measurable with only an hour of incubation.
- Published
- 2016
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