1. Effect of temperature decrease on hemocyte apoptosis of the white shrimp Litopenaeus vannamei.
- Author
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Li, Bin, Xian, Jian-An, Guo, Hui, Wang, An-Li, Miao, Yu-Tao, Ye, Jian-Min, Ye, Chao-Xia, and Liao, Shao-An
- Subjects
WHITELEG shrimp ,APOPTOSIS ,OXIDATIVE stress ,EFFECT of temperature on fishes ,BLOOD cells ,AQUACULTURE & the environment - Abstract
This study investigated the effect of continuous temperature decrease on hemocyte apoptosis of the white shrimp Litopenaeus vannamei. In the stress group, water temperature decreased from 26 to 17 °C at a rate of 1 °C/h. Shrimp kept at 26 ± 0.5 °C were used as control group. Total hemocyte count (THC), reactive oxygen species (ROS) production, cytoplasmic free-Ca (CF-Ca) concentration, mitochondrial membrane potential (MMP), apoptotic cell ratio, and caspase-3 activity of L. vannamei hemocytes were determined when water temperature decreased to 23, 20, and 17 °C, respectively. Increased ROS production in hemocytes was observed when water temperature decreased to 20 and 17 °C. Decreased THC and cellular MMP, increased CF-Ca concentration, apoptotic cell ratio, and caspase-3 activity were shown when water temperature decreased to 17 °C. These results indicate that water temperature decrease can induce oxidative stress on shrimp hemocytes and then cause mitochondria and caspase-3 mediated hemocyte apoptosis and THC reduction, when water temperature decreased to an unconformable level. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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