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Detoxified Jatropha protein isolate ( DJPI), prepared from Jatropha seed cake was hydrolysed using Alcalase 2.4L FG. Both DJPI and hydrolysed DJPI ( HDJPI) were evaluated as a fish meal ( FM) replacement in common carp ( Cyprinus carpio L.) diets. The peptide concentration in HDJPI was determined using matrix-assisted laser desorption ionization-time of flight mass spectrometer ( MALDI- TOF). Twenty-two peptides with molecular mass ranging from m/z 906.455 to 1774.943 Da were identified. Fingerlings (45; av. wt. 10.1 ± 0.4 g) were randomly distributed in five groups with three replicates. An 8-week feeding experiment was conducted to compare the nutritional quality of the HDJPI, DJPI and FM. Fish were fed on iso-nitrogenous diets: control ( FM based protein), J50 (50% of FM protein replaced by DJPI), J75 (75% of FM protein replaced by DJPI), JH50 (50% of FM protein replaced by HDJPI) and JH75 (75% of FM protein replaced by HDJPI). Growth performance was higher for FM fed group compared with the other groups, while nutrient utilization parameters were similar for control and J50 groups and superior ( P < 0.05) to J75 and both the HDJPI fed groups. No variation ( P > 0.05) was observed in blood albumin and hepatosomatic index among all different groups. Blood urea nitrogen, calcium, sodium and potassium ions in the blood were similar among all five groups. [ABSTRACT FROM AUTHOR]