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Bacteria in sediment plays an important role in various biochemical processes in the waters area, for instance degradation of organic matter, biogeochemical cycle, controlling ammonium, nitrates and nitrites concentration, food source for fauna, primary production, and pollution remediation. The purpose of this study was to determine the diversity of bacteria (Genus) in sediment on the Mangrove and Bekantan Conservation Area (KKMB), Tarakan City. The method used was explorative descriptive method with genus identification as parametric test. Bacterial identification parameters were tested using Gram-test, main test and further test was carried out at the Laboratory of Fish Quarantine, Quality Control and Safety Fishery Products Class II Tarakan. The bacteria found in the sediments on the KKMB were 16 genera i.e. Enterobacteria, Eubacterium, Listeria, Actinobacillus, Bacteriodes, Streptococcus, Plesiomonas, Corynebacterium, Pseudomonas, Aeromonas, Bordetella parapertussis, Micrococcus, Staphylococcus, Clostridium, Neisseria, and the dominant numbers found was Bacillus.

Traditional products derived from the local wisdom of the Tarakan City coastal communities, namely in the form of cold protective skin powder from the sun which is made from mangrove raw Sonneratia alba. This product has potential to be developed to improve the economic value of local communities. They can compete with products other sunscreen products produced by industry (commercial) through cream preparation technology (sunscreen).This research was carried out for seven months (February-August 2018) in the Environmental Laboratory of FPIK UBT, Samarinda Pharmacy laboratory (AKFARSAM), and the UNMUL Mathematics and Biochemistry laboratory. The research procedure used maserasi method with 70% ethanol solvent followed by concentrating the extract using a rotary evaporator to obtain extracts of active ingredients and carried out qualitative phytochemical testing. The obtained active ingredient was is then formulated into a sunscreen cream preparation. The level of protection of mixed sunscreen cold powder and commercial cream were tested by using UV-VIS spectrophotometer to see the value of Sun Protection Factor (SPF) at absorbance of 290-320 nm. Qualitative phytochemical test showed that on S.alba contains of positive alkaloids, phenols, flavonoids, steroids, and tannins, and negative saponins. ANOVA statistical analysis (95%) based on the calculated F value