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6 results on '"S. Fattoum"'

1. [Analysis of glycosylated A1c hemoglobin by liquid phase chromatography and immunoagglutination].

Author

Sellami M, Labaied N, Ghanem A, Nagati K, and Fattoum S

Subjects
Agglutination Tests, Case-Control Studies, Humans, Immunologic Tests, Reproducibility of Results, Time Factors, Chromatography, High Pressure Liquid methods, Chromatography, Ion Exchange methods, Glycated Hemoglobin metabolism, Hemoglobinopathies blood
Abstract

Glycated hemoglobin A1c assay is performed using HPLC on cation exchange TSK SP 5 PW column. Separation is obtained in 10 minutes using graduated elution with sodium chloride and Bis-Tris buffer. Hb A1c is resolved from the other hemoglobins (F, A0 and S). Reproductibility of method is quite good and correlated with an immunologic method (DCA 2000).

Published
1995

2. [Identification of abnormal hemoglobins by isoelectric focusing].

Author

Abbes S and Fattoum S

Subjects
Humans, Isoelectric Focusing methods, Hemoglobins, Abnormal analysis
Abstract

Using IEF on slabs of acrylamide gel was adapted for screening of abnormal Hemoglobins which are at the same level by electrophoresis on cellulose acetate strips. This method is fast, inexpensive and allowed the simultaneous analysis of 70 samples of whole blood. The characterization technique of IEF allowed us to distinguish some rare variants like Hb O Arab, HbD and T gamma in B 0-thalassemia.

Published
1984

3. [Double heterozygous Hb O Arab/beta-thalassemia in a Tunisian child].

Author

Haji F, Chadli A, Fattoum S, Souilem J, and Hassine L

Subjects
Adolescent, Fetal Hemoglobin analysis, Hemoglobin A analysis, Hemoglobin A2 analysis, Hemoglobin C analysis, Hemoglobin E analysis, Hemoglobin, Sickle analysis, Hemoglobins, Abnormal analysis, Heterozygote, Humans, Male, Thalassemia blood, Tunisia, Hemoglobins, Abnormal genetics, Thalassemia genetics
Abstract

A study realized in a Tunisian family of 22 persons has revealed a double heterozygoty Hb O Arab/beta(0) thalassemia in a child of 16 years old. The father of this child presents a beta thalassemia and his mother is haemoglobine O Arab homozygote.

Published
1985

4. [Manipulations in genetic engineering. Consequences and fields of application].

Author

Sellami M, Labaied N, and Fattoum S

Subjects
Cloning, Molecular, DNA, Recombinant, Genetic Engineering
Abstract

The genetic engineering permits to produce useful proteins. Authors describe some aspects of cloning strategies and the principal applications in medical, industrial and agricultural domains.

Published
1985

5. [Contribution of hemoglobin synthesis in vitro to the diagnosis of thalassemia].

Author

Sellami M, Abbes S, Guemira F, and Fattoum S

Subjects
Child, Child, Preschool, Humans, In Vitro Techniques, Male, Thalassemia metabolism, Hemoglobins biosynthesis, Reticulocytes metabolism, Thalassemia diagnosis
Abstract

The authors show that the, incubation techniques of reticulocytes, are able to appreciate accurately deficiency of the globin chain synthesis, so they allow to apply certainly diagnosis of thalassemias.

Published
1984

6. [A case of hemoglobin D Punjab in Tunisia. Characterization and structural study].

Author

Guemira F, Hajji F, Sellami M, Abbes S, Ducrocq R, Elion J, and Fattoum S

Subjects
Adult, Chromatography, Chromatography, High Pressure Liquid, Electrophoresis, Genetic Variation, Glutamates, Glutamic Acid, Glutamine, Hemoglobins analysis, Humans, Isoelectric Focusing, Male, Pedigree, Tunisia, Hemoglobins, Abnormal analysis, Hemoglobins, Abnormal genetics
Abstract

Hb D Punjab is a hemoglobin abnormality due to the substitution of a glutamic acid by a glutamic on the hemoglobin beta chain: beta 121 Glu----Gln. Authors report here the first case of this rare variant in a tunisian family.

Published
1987

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