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The conversion of Pampa Biome soils into no-till system for grain production requires acidity correction, at least to fully neutralize exchangeable aluminum (Al). The aim of the study is to evaluate changes in solid phase acidity and Al speciation in soil solution that was conducted to prepare the soil for the implementation of a no-tillage system in a natural pasture area in Southern Brazil. We collected soil samples from the 0–5, 5–10, 10–15 and 15–20 cm layers in soils subjected, or not, to acidity correction to pH 6.0, as well as in soils with, and without, limestone incorporation through plowing and harrowing. Changes in the solid and solution phase attributes, such as soil acidity, were limited to 5 and 10 cm, respectively, 44 months after surface and incorporated liming. Regardless of liming addition to, or lack of thereof in the soil, Al found in the soil solution was mainly associated with organic carbon. This was the main way to decrease plant toxicity under these conditions, which can be supported by plant behavior, especially that of soybean. Liming induced the soil solution Al to exchange its ligand from organic carbon to hydroxyl. [ABSTRACT FROM AUTHOR]