1. Isolation of a new human fetal liver cytochrome P450 cDNA clone: evidence for expression of a limited number of forms of cytochrome P450 in human fetal livers
- Author
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Satoaki Mima, Kanako Nishio, Koshiro Itahashi, Tomomichi Fujitani, Hiroaki Ohi, Masayuki Komori, Mitsukazu Kitada, and Tetsuya Kamataki
- Subjects
Molecular Sequence Data ,Biophysics ,Deoxyribonuclease HindIII ,Biochemistry ,Substrate Specificity ,Cytochrome P-450 Enzyme System ,Complementary DNA ,Gene expression ,Humans ,Genomic library ,Northern blot ,Amino Acid Sequence ,Deoxyribonucleases, Type II Site-Specific ,Molecular Biology ,Antiserum ,Fetus ,biology ,Base Sequence ,cDNA library ,Cytochrome P450 ,Nucleic Acid Hybridization ,DNA ,Molecular biology ,Gene Expression Regulation ,Liver ,embryonic structures ,biology.protein ,DNA Probes - Abstract
From a human fetal liver cDNA library, a new cDNA clone (lambda HFL10) was isolated using an antiserum to P450 HFLa, which has been isolated from livers of human fetuses. Cytochrome P450 cDNAs, namely lambda hPA6, lamda hP2-1, and lambda hPD4 which were highly homologous to cDNA clones, pHY13, Hp1-1, and phP450j, respectively, were also isolated from the cDNA library of human adult livers. Using these cDNA clones as probes together with Lambda HFL10, Northern blot analysis was conducted to determine whether all of these cytochromes were expressed in human fetal livers. The results clearly showed that only P450 HFL10 mRNA was detected in human fetal livers. This result supports the allegation that there is a much more limited number of forms of cytochrome P450 in human fetal livers than in adult livers.
- Published
- 1989