Search

A short-rod-shaped moderately halophilic bacterium, designated CUG 00002, was isolated from the sediment of Xiaochaidan salt lake in Qinghai Province, China by using R2A medium. The cells were Gram-staining negative, aerobic, forming creamy and circular colonies with diameters of 2-3 mm on R2A agar when incubated at 30 °C for 3 days. 16S rRNA gene-based phylogenetic analysis indicated that strain CUG 00002 belonged to the genus Halomonas in the class Gammaproteobacteria, showing highest sequence similarity of 97.1 and 96.7 % to Halomonas mongoliensis Z-7009 (=DSM 17332=VKM B2353) and Halomonas shengliensis SL014B-85 (=CGMCC 1.6444=LMG 23897), respectively. The predominant isoprenoid quinone was ubiquinone-9 (Q9), and the major fatty acids were C, summed feature 3 (comprising C ω7c and/or C ω6c) and summed feature 8 (comprising C ω7c or C ω6c). The genomic DNA G+C content of strain CUG 00002 was 61.8 mol%. The above characteristics were consistent with the placement of the organism in the genus Halomonas. The level of DNA-DNA relatedness between CUG 00002 and its most closely related strain H. mongoliensis Z-7009 was 41.0 ± 1.6 %. Based on the results of phenotypic, phylogenetic and biochemical analyses, strain CUG 00002 represents a novel species of the genus Halomonas, for which the name Halomonas xiaochaidanensis sp. nov. is proposed. The type strain is CUG 00002 (=CCTCC AB 2014152=KCTC 42685). [ABSTRACT FROM AUTHOR]

An aerobic, orange-pigmented, Gram-negative, coccoid bacterium, named CUG 00006, was isolated from the sediment of Gahai Lake, Qinghai Province, China. This organism was alkaline and grew optimally at pH 9 and 20 °C in the presence of 4 % (w/v) NaCl. Strain CUG 00006 contained Q-10 as the major isoprenoid quinone and C as the main fatty acids. The DNA G + C content was 67.8 mol%. The analysis of 16S rRNA gene sequences indicated that strain CUG 00006 was phylogenetically related to members of the genus Paracoccus, with the similarities ranging from 93.5 to 97.9 %. In particular, strain CUG 00006 was closely related to P. marcusii DSM 11574 (97.7 %), P. haeundaensis KCCM 10460 (97.8 %), and P. carotinifaciens IFO 16121 (97.7 %). On the basis of phylogenetic, physiological, and biochemical characterization, strain CUG 00006 is described as a new species of the genus Paracoccus, for which the name Paracoccus gahaiensis sp. nov. is proposed. The type strain is strain CUG 00006 (=CCTCC M 2014217 = KCTC 42687). [ABSTRACT FROM AUTHOR]