1. Identification and characterization of the serine/threonine protein kinases in Bifidobacterium
- Author
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Natalia V. Zakharevich, V. Z. Nezametdinova, D. A. Mavletova, M. G. Alekseeva, Valery N. Danilenko, and Olga V. Averina
- Subjects
Bifidobacterium longum ,Sequence analysis ,Molecular Sequence Data ,ved/biology.organism_classification_rank.species ,Serine threonine protein kinase ,Protein Serine-Threonine Kinases ,medicine.disease_cause ,digestive system ,Biochemistry ,Microbiology ,fluids and secretions ,Bacterial Proteins ,Species Specificity ,Catalytic Domain ,Escherichia coli ,Genetics ,medicine ,Amino Acid Sequence ,Phosphorylation ,Molecular Biology ,Gene ,Phylogeny ,Bifidobacterium ,Bifidobacterium bifidum ,biology ,ved/biology ,Probiotics ,Autophosphorylation ,food and beverages ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,Genes, Bacterial ,bacteria - Abstract
Six genes encoding the bifidobacterial Hanks-type (eukaryote-like) serine/threonine protein kinases (STPK) were identified and classified. The genome of each bifidobacterial strain contains four conserved genes and one species-specific gene. Bifidobacterium longum and Bifidobacterium bifidum possess the unique gene found only in these species. The STPK genes of Russian industrial probiotic strain B. longum B379M were cloned and sequenced. The expression of these genes in Escherichia coli and bifidobacteria was observed. Autophosphorylation of the conserved STPK Pkb5 and species-specific STPK Pkb2 was demonstrated. This is the first report on Hanks-type STPK in bifidobacteria.
- Published
- 2014
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