1. Complete genome sequence of a potyvirus infecting yam beans (Pachyrhizus spp.) in Peru
- Author
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Jan Kreuze, Thomas zum Felde, Bettina Heider, Elisa Romero, Ruby Carolina Tasso, and Segundo Fuentes
- Subjects
Whole genome sequencing ,biology ,Mosaic virus ,Molecular Sequence Data ,Potyvirus ,Pachyrhizus ,Sequence Homology ,General Medicine ,Pachyrrhizus ,Genome, Viral ,Sequence Analysis, DNA ,biology.organism_classification ,Virology ,Genome ,Molecular taxonomy ,Plant virus ,Peru ,Cluster Analysis ,RNA, Viral ,RNA, Small Interfering ,Phylogeny ,Plant Diseases - Abstract
In 2010, yam beans in a field trial in Peru showed viral disease symptoms. Graft-transmission and positive ELISA results using potyvirus-specific antibodies suggested that the symptoms could be the result of a potyviral infection. Small interfering RNA (siRNA) were extracted from one of the samples and sent for high-throughput sequencing. The full genome of a new potyvirus could be assembled from the resulting siRNA sequences, and it was sufficiently different from other sequences to be considered a member of a new species, which we have designated Yam bean mosaic virus (YBMV). Sequence similarity suggests that YBMV has also been detected in yam beans in Indonesia.
- Published
- 2011