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35 results on '"Mizutani, Tetsuya"'

1. Isolation and genetic characterization of novel bovine parechoviruses from Japanese black cattle.

Author

Oba, Mami, Shimotori, Mayo, Teshima, Natsuko, Yamaguchi, Lisa, Takemae, Hitoshi, Sakaguchi, Shoichi, Ishida, Hiroho, Murakami, Hironobu, Mizutani, Tetsuya, and Nagai, Makoto

Abstract

Novel bovine parechoviruses (Bo ParVs) were isolated from the feces of Japanese black cattle. Phylogenetic analysis revealed that the novel Bo ParVs formed an independent cluster, exhibiting 72.2–75.6% nucleotide sequence identity to previous Bo ParVs, suggesting that they represent a new genotype. Bo ParVs, including the novel Bo ParVs, shared sequence similarity with each other in the 3' untranslated region (3'UTR) and exhibited low sequence similarity (<38.9% identity) to other parechoviruses. However, a secondary structure prediction of the 3'UTR revealed that the Bo ParVs shared conserved motifs in domain 2 with parechovirus B and E, suggesting some evolutionary constrains in this region. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Detection and genetic analysis of bovine rhinitis B virus in Japan.

Author

Ishida, Hiroho, Nakamura, Mikari, Murakami, Hironobu, Kazama, Kei, Oba, Mami, Takemae, Hitoshi, Mizutani, Tetsuya, Ouchi, Yoshinao, Kawakami, Junko, Tsuzuku, Satoko, and Nagai, Makoto

Subjects
RHINITIS, WHOLE genome sequencing, AMINO acid sequence, MANNHEIMIA haemolytica, GENETIC variation, BOS, MOSAIC viruses
Abstract

Bovine rhinitis B virus (BRBV) (genus Aphthovirus, family Picornaviridae) is a significant etiological agent of the bovine respiratory disease complex. Despite global reports on BRBV, genomic data for Japanese strains are not available. In this study, we aimed to obtain genomic information on BRBV in Japan and analyze its genetic characteristics. In nasal swabs from 66 cattle, BRBV was detected in 6 out of 10 symptomatic and 4 out of 56 asymptomatic cattle. Using metagenomic sequencing and Sanger sequencing, the nearly complete genome sequences of two Japanese BRBV strains, IBA/2211/2 and LAV/238002, from symptomatic and asymptomatic cattle, respectively, were determined. These viruses shared significant genetic similarity with known BRBV strains and exhibited unique mutations and recombination events, indicating dynamic evolution, influenced by regional environmental and biological factors. Notably, the leader gene was only approximately 80% and 90% identical in its nucleotide and amino acid sequence, respectively, to all of the BRBV strains with sequences in the GenBank database, indicating significant genetic divergence in the Japanese BRBV leader gene. These findings provide insights into the genetic makeup of Japanese BRBV strains, enriching our understanding of their genetic diversity and evolutionary mechanisms. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Genomic diversity of group A rotaviruses from wild boars and domestic pigs in Japan: wide prevalence of NSP5 carrying the H2 genotype.

Author

Shizawa, Shigeki, Fukuda, Fujiko, Kikkawa, Yasuhiro, Oi, Toru, Takemae, Hitoshi, Masuda, Tsuneyuki, Ishida, Hiroho, Murakami, Hironobu, Sakaguchi, Shoichi, Mizutani, Tetsuya, Nagai, Makoto, and Oba, Mami

Abstract

Group A rotavirus (RVA) sequences were detected in 10.8% (23/212) and 20.7% (87/421) of fecal samples collected in 2017-2022 from wild boars and domestic pigs, using next-generation sequencing. Complete genome sequence analysis of one wild boar and 13 domestic pig RVAs revealed that six of them carried the rare H2 NSP5 genotype. Out of the 39 samples for which the NSP5 genotype could be determined, 23 (59.0%) were of genotype H2. H2 porcine RVAs consist exclusively of Japanese porcine RVAs and exhibit sequence diversity in each segment, suggesting that H2 porcine RVAs may have evolved through reassortment within the Japanese pig population. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Genetic diversity, reassortment, and recombination of mammalian orthoreoviruses from Japanese porcine fecal samples

Author

Fukase, Yuka, primary, Minami, Fujiko, additional, Masuda, Tsuneyuki, additional, Oi, Toru, additional, Takemae, Hitoshi, additional, Ishida, Hiroho, additional, Murakami, Hironobu, additional, Aihara, Naoyuki, additional, Shiga, Takanori, additional, Kamiie, Junichi, additional, Furuya, Tetsuya, additional, Mizutani, Tetsuya, additional, Oba, Mami, additional, and Nagai, Makoto, additional

Published
2022
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8. Multiple genotypes of enterovirus G carrying a papain-like cysteine protease (PL-CP) sequence circulating on two pig farms in Japan: first identification of enterovirus G10 carrying a PL-CP sequence

Author

Sekiguchi, Yuya, primary, Nagata, Ayaka, additional, Sunaga, Fujiko, additional, Oi, Toru, additional, Imai, Ryo, additional, Madarame, Hiroo, additional, Katayama, Yukie, additional, Oba, Mami, additional, Okabayashi, Tamaki, additional, Misawa, Naoaki, additional, Oka, Tomoichiro, additional, Mizutani, Tetsuya, additional, and Nagai, Makoto, additional

Published
2020
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10. Complete genome sequencing and genetic analysis of a Japanese porcine torovirus strain detected in swine feces

Author

Fujii, Yuki, primary, Kashima, Yuki, additional, Sunaga, Fujiko, additional, Aoki, Hiroshi, additional, Imai, Ryo, additional, Sano, Kaori, additional, Katayama, Yukie, additional, Omatsu, Tsutomu, additional, Oba, Mami, additional, Furuya, Tetsuya, additional, Tsuzuku, Satoko, additional, Ouchi, Yoshinao, additional, Shirai, Junsuke, additional, Mizutani, Tetsuya, additional, Oka, Tomoichiro, additional, and Nagai, Makoto, additional

Published
2019
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11. Molecular characterization of feline paramyxovirus in Japanese cat populations

Author

Sakaguchi, Shoichi, primary, Nakagawa, So, additional, Mitsuhashi, Satomi, additional, Ogawa, Makoto, additional, Sugiyama, Kazutoshi, additional, Tamukai, Kenichi, additional, Koide, Rie, additional, Katayama, Yukie, additional, Nakano, Takashi, additional, Makino, Shinji, additional, Imanishi, Tadashi, additional, Miyazawa, Takayuki, additional, and Mizutani, Tetsuya, additional

Published
2019
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12. Characterization of cross-clade monoclonal antibodies against H5N1 highly pathogenic avian influenza virus and their application to the antigenic analysis of diverse H5 subtype viruses

Author

Gronsang, Dulyatad, Bui, Anh N., Trinh, Dai Q., Bui, Vuong N., Nguyen, Khong V., Can, Minh X., Omatsu, Tsutomu, Mizutani, Tetsuya, Nagai, Makoto, Katayama, Yukie, Thampaisarn, Rapeewan, Ogawa, Haruko, and Imai, Kunitoshi

Subjects
virus diseases
Abstract

application/pdf, H5N1 highly pathogenic avian influenza viruses (HPAIVs) are a threat to both animal and public health and require specific and rapid detection for prompt disease control. We produced three neutralizing anti-hemagglutinin (HA) monoclonal antibodies (mAbs) using two clades (2.2 and 2.5) of the H5N1 HPAIV isolated in Japan. Blocking immunofluorescence tests showed that each mAb recognized different epitopes; 3B5.1 and 3B5.2 mAbs against the clade 2.5 virus showed cross-clade reactivity to all 26 strains from clades 1, 2.2, 2.3.2.1, 2.3.2.1a, b, c and 2.3.4, suggesting that the epitope(s) recognized are conserved. Conversely, the 1G5 mAb against the clade 2.2 virus showed reactivity to only clades 1, 2.3.4 and 2.5 strains. An analysis of escape mutants, and some clades of the H5N1 viruses recognized by 3B5.1 and 3B5.2 mAbs, suggested that the mAbs bind to an epitope, including amino acid residues at position 162 in the HA1 protein (R162 and K162). Unexpectedly, however, when five Eurasian-origin H5 low-pathogenic AIV (LPAIV) strains with R162 were examined (EA-nonGsGD clade) as well as two American-origin strains (Am-nonGsGD clade), the mAb recognized only EA-nonGsGD clade strains. The R162 and K162 residues in the HA1 protein were highly conserved among 36 of the 43 H5N1 clades reported, including clades 2.3.2.1a and 2.3.2.1c that are currently circulating in Asia, Africa and Europe. The amino acid residues (158-PTIKRSYNNTNQE-170) in the HA1 protein are probably an epitope responsible for the cross-clade reactivity of the mAbs, considering the epitopes reported elsewhere. The 3B5.1 and 3B5.2 mAbs may be useful for the specific detection of H5N1 HPAIVs circulating in the field.

Published
2017

13. Phylogenetic analysis of novel posaviruses detected in feces of Japanese pigs with posaviruses and posa-like viruses of vertebrates and invertebrates

Author

Aoki, Hiroshi, primary, Sunaga, Fujiko, additional, Ochiai, Hideharu, additional, Masuda, Tsuneyuki, additional, Ito, Mika, additional, Akagami, Masataka, additional, Naoi, Yuki, additional, Sano, Kaori, additional, Katayama, Yukie, additional, Omatsu, Tsutomu, additional, Oba, Mami, additional, Sakaguchi, Shoichi, additional, Furuya, Tetsuya, additional, Ouchi, Yoshinao, additional, Shirai, Junsuke, additional, Mizutani, Tetsuya, additional, Oka, Tomoichiro, additional, and Nagai, Makoto, additional

Published
2019
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14. Complete genome sequencing and genetic analysis of a Japanese porcine torovirus strain detected in swine feces.

Author

Fujii, Yuki, Kashima, Yuki, Sunaga, Fujiko, Aoki, Hiroshi, Imai, Ryo, Sano, Kaori, Katayama, Yukie, Omatsu, Tsutomu, Oba, Mami, Furuya, Tetsuya, Tsuzuku, Satoko, Ouchi, Yoshinao, Shirai, Junsuke, Mizutani, Tetsuya, Oka, Tomoichiro, and Nagai, Makoto

Subjects
NUCLEOTIDE sequencing, SEQUENCE analysis, SWINE, GENETIC recombination, CHLOROPLAST DNA, PORCINE reproductive & respiratory syndrome
Abstract

We sequenced the complete genome of a porcine torovirus (PToV) strain from Japan for the first time. Whole-genome analysis revealed that this strain (Iba/2018) has a mosaic sequence composed of at least three genome backgrounds, related to US, Chinese and German PToV strains. Clear recombination breakpoints were detected in the M and HE coding regions. A similarity plot and structural analysis demonstrated that the HE coding region exhibits the highest diversity, and the most sequence variation was found in the lectin domain. PToVs were divided into two lineages in the HE region, whereas clear lineages were not found in other regions. [ABSTRACT FROM AUTHOR]

Published
2020
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15. Molecular characterization of feline paramyxovirus in Japanese cat populations.

Author

Sakaguchi, Shoichi, Nakagawa, So, Mitsuhashi, Satomi, Ogawa, Makoto, Sugiyama, Kazutoshi, Tamukai, Kenichi, Koide, Rie, Katayama, Yukie, Nakano, Takashi, Makino, Shinji, Imanishi, Tadashi, Miyazawa, Takayuki, and Mizutani, Tetsuya

Subjects
CATS, PARAMYXOVIRUSES, URINE, GENES, CHLOROPLAST DNA
Abstract

Feline paramyxovirus (FPaV) is a member of the family Paramyxoviridae that has been reported only in Germany and the United Kingdom. We detected FPaV for the first time in Japan by transcriptome sequencing of cat urine samples. We determined the genome structure of FPaV and conducted a phylogenetic analysis. It was found that FPaV belongs to the genus Jeilongvirus and forms a clade with Mount Mabu Lophuromys virus 1 (MMLV-1). FPaV lacks a small hydrophobic (SH) gene that is found in members of the genus Jeilongvirus; however, some jeilongviruses also do not have this gene. These results provide information about the diversity and evolution of paramyxoviruses. [ABSTRACT FROM AUTHOR]

Published
2020
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21. First isolation and characterization of pteropine orthoreoviruses in fruit bats in the Philippines

Author

Taniguchi, Satoshi, primary, Maeda, Ken, additional, Horimoto, Taisuke, additional, Masangkay, Joseph S., additional, Puentespina, Roberto, additional, Alvarez, James, additional, Eres, Eduardo, additional, Cosico, Edison, additional, Nagata, Noriyo, additional, Egawa, Kazutaka, additional, Singh, Harpal, additional, Fukuma, Aiko, additional, Yoshikawa, Tomoki, additional, Tani, Hideki, additional, Fukushi, Shuetsu, additional, Tsuchiaka, Shinobu, additional, Omatsu, Tsutomu, additional, Mizutani, Tetsuya, additional, Une, Yumi, additional, Yoshikawa, Yasuhiro, additional, Shimojima, Masayuki, additional, Saijo, Masayuki, additional, and Kyuwa, Shigeru, additional

Published
2017
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22. Identification of further diversity among posaviruses

Author

Sano, Kaori, primary, Naoi, Yuki, additional, Kishimoto, Mai, additional, Masuda, Tsuneyuki, additional, Tanabe, Hitomi, additional, Ito, Mika, additional, Niira, Kazutaka, additional, Haga, Kei, additional, Asano, Keigo, additional, Tsuchiaka, Shinobu, additional, Omatsu, Tsutomu, additional, Furuya, Tetsuya, additional, Katayama, Yukie, additional, Oba, Mami, additional, Ouchi, Yoshinao, additional, Yamasato, Hiroshi, additional, Ishida, Motohiko, additional, Shirai, Junsuke, additional, Katayama, Kazuhiko, additional, Mizutani, Tetsuya, additional, and Nagai, Makoto, additional

Published
2016
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24. Characterization and phylogenetic analysis of a novel picornavirus from swine feces in Japan

Author

Naoi, Yuki, primary, Kishimoto, Mai, additional, Masuda, Tsuneyuki, additional, Ito, Mika, additional, Tsuchiaka, Shinobu, additional, Sano, Kaori, additional, Yamasato, Hiroshi, additional, Omatsu, Tsutomu, additional, Aoki, Hiroshi, additional, Furuya, Tetsuya, additional, Katayama, Yukie, additional, Oba, Mami, additional, Okada, Takashi, additional, Shirai, Junsuke, additional, Mizutani, Tetsuya, additional, and Nagai, Makoto, additional

Published
2016
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26. Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bovine astrovirus

Author

Nagai, Makoto, primary, Omatsu, Tsutomu, additional, Aoki, Hiroshi, additional, Otomaru, Konosuke, additional, Uto, Takehiko, additional, Koizumi, Motoya, additional, Minami-Fukuda, Fujiko, additional, Takai, Hikaru, additional, Murakami, Toshiaki, additional, Masuda, Tsuneyuki, additional, Yamasato, Hiroshi, additional, Shiokawa, Mai, additional, Tsuchiaka, Shinobu, additional, Naoi, Yuki, additional, Sano, Kaori, additional, Okazaki, Sachiko, additional, Katayama, Yukie, additional, Oba, Mami, additional, Furuya, Tetsuya, additional, Shirai, Junsuke, additional, and Mizutani, Tetsuya, additional

Published
2015
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28. The complete genomic sequence of Rhinolophus gammaherpesvirus 1 isolated from a greater horseshoe bat.

Author

Noguchi, Keita, Kuwata, Ryusei, Shimoda, Hiroshi, Mizutani, Tetsuya, Hondo, Eiichi, and Maeda, Ken

Subjects
HORSESHOE bats, HERPESVIRUSES, CELL lines, HOMOLOGY (Biology), GENOMICS
Abstract

In a comprehensive research project on bat viruses, we successfully isolated a novel herpesvirus from the spleen of a greater horseshoe bat (Rhinolophus ferrumequinum) in Japan using a cell line established from the kidney of the same bat. This herpesvirus was a novel gammaherpesvirus (Rhinolophus gammaherpesvirus 1; RGHV-1), which belonged to the genus Percavirus. The whole RGHV-1 genome (147,790 bp) showed that 12 of the 84 genes predicted to contain open reading frames did not show any homology to those of other herpesviruses. [ABSTRACT FROM AUTHOR]

Published
2019
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29. Characterization of a genetic and antigenic variant of avian paramyxovirus 6 isolated from a migratory wild bird, the red-necked stint (Calidris ruficollis)

Author

Bui, Vuong Nghia, primary, Mizutani, Tetsuya, additional, Nguyen, Tung Hoang, additional, Trinh, Dai Quang, additional, Awad, Sanaa S. A., additional, Minoungou, Germaine L., additional, Yamamoto, Yu, additional, Nakamura, Kikuyasu, additional, Saito, Keisuke, additional, Watanabe, Yukiko, additional, Runstadler, Jonathan, additional, Huettmann, Falk, additional, Ogawa, Haruko, additional, and Imai, Kunitoshi, additional

Published
2014
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31. Characterization of Dak Nong virus, an insect nidovirus isolated from Culex mosquitoes in Vietnam

Author

Kuwata, Ryusei, primary, Satho, Tomomitsu, additional, Isawa, Haruhiko, additional, Yen, Nguyen Thi, additional, Phong, Tran Vu, additional, Nga, Phan Thi, additional, Kurashige, Tomokazu, additional, Hiramatsu, Yukihiro, additional, Fukumitsu, Yuki, additional, Hoshino, Keita, additional, Sasaki, Toshinori, additional, Kobayashi, Mutsuo, additional, Mizutani, Tetsuya, additional, and Sawabe, Kyoko, additional

Published
2013
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32. Genomic and serological detection of bat coronavirus from bats in the Philippines

Author

Tsuda, Shumpei, primary, Watanabe, Shumpei, additional, Masangkay, Joseph S., additional, Mizutani, Tetsuya, additional, Alviola, Phillip, additional, Ueda, Naoya, additional, Iha, Koichiro, additional, Taniguchi, Satoshi, additional, Fujii, Hikaru, additional, Kato, Kentaro, additional, Horimoto, Taisuke, additional, Kyuwa, Shigeru, additional, Yoshikawa, Yasuhiro, additional, and Akashi, Hiroomi, additional

Published
2012
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33. Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0)

Author

Yamao, Takuya, primary, Eshita, Yuki, additional, Kihara, Yuki, additional, Satho, Tomomitsu, additional, Kuroda, Makoto, additional, Sekizuka, Tsuyoshi, additional, Nishimura, Miho, additional, Sakai, Kouji, additional, Watanabe, Shumpei, additional, Akashi, Hiroomi, additional, Rongsriyam, Yupha, additional, Komalamisra, Narumon, additional, Srisawat, Raweewan, additional, Miyata, Takeshi, additional, Sakata, Akira, additional, Hosokawa, Masato, additional, Nakashima, Manabu, additional, Kashige, Nobuhiro, additional, Miake, Fumio, additional, Fukushi, Shuetsu, additional, Nakauchi, Mina, additional, Saijo, Masayuki, additional, Kurane, Ichiro, additional, Morikawa, Shigeru, additional, and Mizutani, Tetsuya, additional

Published
2008
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34. Existence of feline morbillivirus infection in Japanese cat populations.

Author

Furuya, Tetsuya, Sassa, Yukiko, Omatsu, Tsutomu, Nagai, Makoto, Fukushima, Ryuji, Shibutani, Makoto, Yamaguchi, Tomohiro, Uematsu, Yosuke, Shirota, Kinji, and Mizutani, Tetsuya

Subjects
MORBILLIVIRUSES, PARAMYXOVIRUSES, NUCLEOTIDE sequence, CATS, NEPHRITIS, PATHOLOGY -- Examinations, questions, etc., DIAGNOSIS
Abstract

Feline morbillivirus (FmoPV) is a member of a new virus species that has only been found in the Hong Kong cat population. For the first time, however, we have now detected nucleotide sequences similar to FmoPV in samples from Japanese cat populations. The positive rates for urine and blood samples from Japanese cats were 6.1 % (5/82) and 10 % (1/10), respectively. These sequences are similar to the previously reported FmoPV, with 92-94 % identity, and substantially different from all other morbilliviruses. Phylogenetic analysis of the identified Japanese FmoPVs and other morbilliviruses demonstrated a pattern similar to those previously published for the FmoPV viruses isolated in Hong Kong. FmoPV RNA was also detected from formalin-fixed paraffin-embedded (FFPE) kidney tissues of cats with nephritis, with a positive rate of 40 % (4/10). By using nested-set primers based on the FmoPV sequence and RNA from FFPE tissues, we demonstrated the existence of FmoPV infection in Japanese cats and established the method for detection of the FmoPV RNA from kidney tissues prepared for pathology examinations, which is useful for studies on the pathogenicity of the virus. [ABSTRACT FROM AUTHOR]

Published
2014
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35. Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0).

Author

Yamao T, Eshita Y, Kihara Y, Satho T, Kuroda M, Sekizuka T, Nishimura M, Sakai K, Watanabe S, Akashi H, Rongsriyam Y, Komalamisra N, Srisawat R, Miyata T, Sakata A, Hosokawa M, Nakashima M, Kashige N, Miake F, Fukushi S, Nakauchi M, Saijo M, Kurane I, Morikawa S, and Mizutani T

Subjects
Amino Acid Sequence, Animals, Base Sequence, Bunyaviridae classification, Chlorocebus aethiops, DNA, Complementary chemistry, Larva virology, Molecular Sequence Data, Nucleic Acid Amplification Techniques methods, Phylogeny, RNA, Viral chemistry, Vero Cells, Aedes virology, Bunyaviridae genetics, Bunyaviridae isolation & purification, RNA, Viral genetics, Virology methods
Abstract

In this study, we improved a method for rapid determination of viral RNA sequences (RDV) to overcome the limitations of previous versions. The RDV ver4.0 method can detect RNA sequences with at least 1,000 copies as starting material. A novel virus, which was isolated from field-collected Aedes aegypti larvae in the Phasi Charoen district of Thailand using C6/36 cells, was identified using the RDV ver4.0 protocol. The virus was named Phasi Charoen virus (PhaV). We used a high-throughput pyrosequencing approach to obtain more information about the genome sequence of PhaV. Analysis of a phylogenic tree based on amino acid sequences strongly suggested that PhaV belongs to the family Bunyaviridae.

Published
2009
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