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4. An acyclovir-resistant strain of herpes simplex virus type 2 which is highly virulent for mice.

Author

Sakuma S, Yamamoto M, Kumano Y, and Mori R

Subjects
Animals, Autoradiography, DNA Restriction Enzymes, DNA, Viral analysis, Drug Resistance, Microbial genetics, Female, Ganglia, Spinal microbiology, Herpes Simplex drug therapy, Humans, Mice, Mice, Inbred BALB C, Microbial Sensitivity Tests, Simplexvirus genetics, Simplexvirus pathogenicity, Species Specificity, Thymidine Kinase metabolism, Vidarabine pharmacology, Viral Plaque Assay, Virulence, Virus Cultivation, Acyclovir pharmacology, Herpes Simplex microbiology, Simplexvirus drug effects
Abstract

Herpes simplex virus type 2 (HSV-2), strain YS-4 C-1, isolated by plaque cloning from a clinical isolate was found to be resistant to acyclovir (ACV; acycloguanosine) in vitro. It was sensitive to phosphonoacetic acid and 9-beta-D-arabinofuranosyladenine. Thymidine kinase (TK) activity of YS-4 C-1 was less than 1% of that of other strains from the same clinical source. However, thymidine plaque autoradiography showed that YS-4 C-1 was not completely deficient in TK activity. YS-4 C-1 showed high virulence for mice like other HSV-2 strains which were sensitive to ACV. YS-4 C-1 was able to establish latent infection in mice. Virus isolated from the brain of a mouse died after being inoculated with YS-4 C-1 was also resistant to ACV. ACV was not effective in mice inoculated with YS-4 C-1. This study shows that not all ACV-resistant strains are avirulent for mice.

Published
1988
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