1. Antiangiogenic activity of semisynthetic biotechnological heparins: low-molecular-weight-sulfated Escherichia coli K5 polysaccharide derivatives as fibroblast growth factor antagonists.
- Author
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Presta M, Oreste P, Zoppetti G, Belleri M, Tanghetti E, Leali D, Urbinati C, Bugatti A, Ronca R, Nicoli S, Moroni E, Stabile H, Camozzi M, Hernandez GA, Mitola S, Dell'Era P, Rusnati M, and Ribatti D
- Subjects
- Angiogenesis Inhibitors genetics, Animals, Bacterial Capsules, CHO Cells chemistry, CHO Cells metabolism, Cattle, Cell Adhesion physiology, Cell Line, Cell Proliferation drug effects, Chick Embryo, Chorioallantoic Membrane drug effects, Cricetinae, Cricetulus, Endothelial Cells chemistry, Endothelial Cells metabolism, Escherichia coli genetics, Fibroblast Growth Factor 2 analogs & derivatives, Fibroblast Growth Factor 2 metabolism, Fibroblast Growth Factors analogs & derivatives, Fibroblast Growth Factors metabolism, Heparan Sulfate Proteoglycans analogs & derivatives, Heparan Sulfate Proteoglycans deficiency, Heparan Sulfate Proteoglycans metabolism, Heparin, Low-Molecular-Weight chemical synthesis, Heparin, Low-Molecular-Weight genetics, Integrin alphaVbeta3 metabolism, Mice, Neovascularization, Physiologic drug effects, Polysaccharides, Bacterial genetics, Angiogenesis Inhibitors biosynthesis, Escherichia coli chemistry, Fibroblast Growth Factor 2 antagonists & inhibitors, Genetic Engineering methods, Heparin, Low-Molecular-Weight biosynthesis, Polysaccharides, Bacterial biosynthesis
- Abstract
Objective: Low-molecular-weight heparin (LMWH) exerts antitumor activity in clinical trials. The K5 polysaccharide from Escherichia coli has the same structure as the heparin precursor. Chemical and enzymatic modifications of K5 polysaccharide lead to the production of biotechnological heparin-like compounds. We investigated the fibroblast growth factor-2 (FGF2) antagonist and antiangiogenic activity of a series of LMW N,O-sulfated K5 derivatives., Methods and Results: Surface plasmon resonance analysis showed that LMW-K5 derivatives bind FGF2, thus inhibiting its interaction with heparin immobilized to a BIAcore sensor chip. Interaction of FGF2 with tyrosine-kinase receptors (FGFRs), heparan sulfate proteoglycans (HSPGs), and alpha(v)beta3 integrin is required for biological response in endothelial cells. Similar to LMWH, LMW-K5 derivatives abrogate the formation of HSPG/FGF2/FGFR ternary complexes by preventing FGF2-mediated attachment of FGFR1-overexpressing cells to HSPG-bearing cells and inhibit FGF2-mediated endothelial cell proliferation. However, LMW-K5 derivatives, but not LMWH, also inhibit FGF2/alpha(v)beta3 integrin interaction and consequent FGF2-mediated endothelial cell sprouting in vitro and angiogenesis in vivo in the chick embryo chorioallantoic membrane., Conclusions: LMW N,O-sulfated K5 derivatives affect both HSPG/FGF2/FGFR and FGF2/alpha(v)beta3 interactions and are endowed with FGF2 antagonist and antiangiogenic activity. These compounds may provide the basis for the design of novel LMW heparin-like angiostatic compounds.
- Published
- 2005
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