1. Osteoarthritic lesions: involvement of three different collagenases.
- Author
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Shlopov BV, Lie WR, Mainardi CL, Cole AA, Chubinskaya S, and Hasty KA
- Subjects
- Aged, Animals, Base Sequence, Blotting, Northern, Collagenases biosynthesis, Collagenases genetics, DNA Primers chemistry, Gene Expression drug effects, Humans, Interleukin-1 pharmacology, Male, Matrix Metalloproteinase 1, Matrix Metalloproteinase 13, Matrix Metalloproteinase 3 genetics, Matrix Metalloproteinase 8, Middle Aged, Osteoarthritis genetics, Polymerase Chain Reaction, RNA, Messenger metabolism, Swine, Tretinoin pharmacology, Tumor Necrosis Factor-alpha pharmacology, Cartilage, Articular enzymology, Collagenases analysis, Osteoarthritis enzymology, Osteoarthritis pathology
- Abstract
Objective: To assess the presence of fibroblast collagenase (MMP-1), neutrophil collagenase (MMP-8), and collagenase 3 (MMP-13) in osteoarthritic (OA) cartilage, with particular emphasis on areas of macroscopic cartilage erosion., Methods: Messenger RNA (mRNA) levels were assessed by reverse transcriptase-polymerase chain reaction (RT-PCR), in situ hybridization, and Northern blot analysis., Results: MMP-1 and MMP-13 were expressed at higher levels by OA chondrocytes than by normal chondrocytes. In addition, mRNA for MMP-8 was present in OA cartilage but not normal cartilage by PCR and Northern blot analyses. Chondrocytes from areas surrounding the OA lesion expressed greater quantities of MMP-1 and MMP-13 compared with normal chondrocytes, suggesting local modulation by mechanical and inflammatory factors. Tumor necrosis factor alpha stimulated the expression of all 3 collagenases. Retinoic acid, an agent which induces autodigestion of cartilage in vitro, stimulated only the expression of MMP-13., Conclusion: These findings suggest a key role of MMP-13 and MMP-8, as well as MMP-1 in osteoarthritis.
- Published
- 1997
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