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Jinhongtang is a traditional Chinese medicine formula composed of Rheum palmatum L. stem, Sargentodoxa cuneata stem, and Taraxacum mongolicum and is used for the treatment of sepsis. However, quality assessment method for Jinhongtang is not available. In present study, we developed a UFLC-MS/MS method to determine 16 analytes in 20 batches of home-made and commercial Jinhongtang. Multivariate statistical analysis revealed the significant differences in the quality of home-made and commercial Jinhongtang and the difference in the quality of home-made samples was more significant. The integrated strategy based on UFLC-MS/MS and multivariate statistical analysis provided a new basis for the overall quality assessment of Jinhongtang. [ABSTRACT FROM AUTHOR]