1. Effect of Dipeptides on Maturation, Fertilization and Subsequent Embryonic Development of Porcine Oocytes
- Author
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K. M. A. Tareq, Quzi Sharmin Akter, Hirotada Tsujii, M. A. M. Yahia Khandoker, and Inho Choi
- Subjects
Ammonia Accumulation ,Dipeptides ,Incorporation and Oxidation ,Porcine Embryos ,Animal culture ,SF1-1100 ,Animal biochemistry ,QP501-801 - Abstract
The effects of amino acids and dipeptides on in vitro production of porcine embryos and accumulation of ammonia in culture medium during developmental stages were examined in this study. The maturation, fertilization and development of embryonic cultures were performed in modified Tissue culture medium (mTCM)-199 supplemented with 10% (v/v) porcine follicular fluid, modified Tyrode’s albumin lactate pyruvate (mTALP) medium, and modified North Carolina State University (mNCSU)-23 medium, respectively. In addition, amino acids and dipeptides of different concentrations and combinations were used to treat the embryos. The addition of L-alanyl-L-glutamine (AlnGln)+L-glycyl-L-glutamine (GlyGln) significantly (p
- Published
- 2013
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