1. Influence of different presentation forms of chlorhexidine on contaminated root canals during agitation.
- Author
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Lopes ABS, R Lima A, D Bronzato J, Herrera DR, Francisco PA, Carvalho MCC, Abuna G, Sinhoreti M, and Gomes BPFA
- Subjects
- Humans, Root Canal Preparation methods, Root Canal Preparation instrumentation, Colony Count, Microbial, Anti-Infective Agents, Local pharmacology, Anti-Infective Agents, Local therapeutic use, Therapeutic Irrigation methods, Sonication methods, Disinfection methods, Gels, Bicuspid, Chlorhexidine pharmacology, Chlorhexidine therapeutic use, Root Canal Irrigants pharmacology, Root Canal Irrigants therapeutic use, Dental Pulp Cavity microbiology, Enterococcus faecalis drug effects, Microscopy, Confocal, Dentin drug effects, Dentin microbiology
- Abstract
This in vitro study aimed to verify the influence of chlorhexidine (CHX) (gel and solution) in association with different activation protocols on disinfection of root canals contaminated with Enterococcus faecalis. In total, 120 lower premolar roots were selected, contaminated and divided into 12 groups according to irrigation technique and substance. Samples were collected before and after each irrigation technique and analysed for colony-forming units (CFU). Three dentin discs were obtained for quantification of viable intratubular cells by using confocal laser scanning microscopy (CLSM). CFU results showed that sonic and ultrasonic were more effective than conventional irrigation. Ultrasonic activation was more effective than sonic for CHX gel (p < 0.05). CLSM showed that either activation was better than conventional in all groups and root canal thirds (p < 0.05). It was concluded that ultrasonic/sonic activation was more effective than conventional techniques and CHX enhanced intratubular dentin decontamination across all irrigation methods., (© 2024 Australian Society of Endodontology Inc.)
- Published
- 2024
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