1. Neuroprotective effect of three caffeic acid derivatives via ameliorate oxidative stress and enhance PKA/CREB signaling pathway
- Author
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Hengyi Yu, Hongyan Wang, Yongfang Lei, Qian Chen, Wei Fu, and Xiuhua Ren
- Subjects
0301 basic medicine ,Antioxidant ,Curcumin ,Cell Survival ,medicine.medical_treatment ,CREB ,medicine.disease_cause ,Neuroprotection ,PC12 Cells ,03 medical and health sciences ,Behavioral Neuroscience ,chemistry.chemical_compound ,Mice ,Random Allocation ,0302 clinical medicine ,Caffeic Acids ,medicine ,Caffeic acid ,Avoidance Learning ,Animals ,Caffeic acid phenethyl ester ,Protein kinase A ,Cyclic AMP Response Element-Binding Protein ,Maze Learning ,Spatial Memory ,biology ,Dose-Response Relationship, Drug ,Galactose ,Hydrogen Peroxide ,Phenylethyl Alcohol ,Cyclic AMP-Dependent Protein Kinases ,Rats ,Oxidative Stress ,030104 developmental biology ,Neuroprotective Agents ,chemistry ,Biochemistry ,biology.protein ,Lactates ,030217 neurology & neurosurgery ,Oxidative stress ,Signal Transduction - Abstract
This study was conducted to elucidate the neuroprotective effect of caffeic acid phenethyl ester (CAPE), (R)-2-Hydroxy-3-(3,4-dihydroxyphenyl) propionic acid (Danshensu) and Curcumin, three caffeic acid derivatives which are contained in fruits, grains and certain dietary supplements. Our results showed that these compounds significantly attenuated H2O2-induced toxicity in PC12 cells in a dose-dependent manner. Furthermore, these compounds significantly improved the behavioral performance of d-gal-treated mice in both step-down avoidance test and Morris water maze test. Biochemical examination and western blot analysis showed that these compounds could ameliorate oxidative stress and facilitate activation of the protein kinase A (PKA)-cyclic AMP response element-binding protein (CREB) pathway. Its beneficial effects may partly relate to enhancing the activity of endogenous antioxidant enzymes and modulating the PKA/CREB signaling pathway. Furthermore, our results also indicated that the presence of 3, 4-dihydroxyphenyl groups in A ring may enhance their neuroprotective activity.
- Published
- 2017