1. Evaluation of the potential anti-allergic effects of heat-inactivated Lactobacillus paracasei V0151 in vitro, ex vivo, and in vivo.
- Author
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Liu YW, Fu TY, Peng WS, Chen YH, Cao YM, Chen CC, Hung WL, and Tsai YC
- Subjects
- Animals, Cells, Cultured, Cytokines metabolism, Leukocytes, Mononuclear immunology, Macrophages immunology, Mice, Inbred BALB C, Nitric Oxide metabolism, Nitric Oxide Synthase Type II biosynthesis, Anti-Allergic Agents pharmacology, Hot Temperature, Lactobacillus immunology, Lactobacillus radiation effects
- Abstract
The efficacy of Lactobacillus paracasei V0151 (V0151), isolated from the faeces of a child, to modulate immune responses was investigated. In RAW 264.7 cells expressing an inducible nitric oxide synthase (iNOS)-directed luciferase gene, heat-inactivated V0151 stimulated iNOS expression followed by nitric oxide production. V0151 significantly elevated interferon gamma, interleukin (IL)-10, tumour necrosis factor alpha, and IL-1β production in human peripheral blood mononuclear cells. In splenocytes isolated from ovalbumin (OVA)-sensitised BALB/c mice treated with OVA and V0151 at different bacterium-to-cell ratios (1:1, 10:1, and 20:1) for 96 h, IL-2, IL-4, IL-5, and IL-13 production was dose-dependently downregulated, whereas IL-12 was dose-dependently upregulated. Collectively, our findings indicate that V0151 might regulate pro-inflammatory factors in macrophages and splenocytes. Furthermore, the T helper 1/T helper 2 (Th1/Th2) balance was also skewed toward Th1 dominance through the elevation of Th1 cytokine production.
- Published
- 2015
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