1. The SCF ubiquitin ligase Slimb controls Nerfin-1 turnover in Drosophila.
- Author
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Lin, Xiaohui, Wang, Feng, Li, Yuanpei, Zhai, Chaojun, Wang, Guiping, Zhang, Xiaoting, Gao, Yang, Yi, Tao, Sun, Dan, and Wu, Shian
- Subjects
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DROSOPHILA melanogaster genetics , *UBIQUITIN ligases , *ZINC-finger proteins , *HOMOLOGY (Biochemistry) , *NEURAL physiology , *NEUROENDOCRINE tumors - Abstract
The C2H2 type zinc-finger transcription factor Nerfin-1 expresses dominantly in Drosophila nervous system and plays an important role in early axon guidance decisions and preventing neurons dedifferentiation. Recently, increasing reports indicated that INSM1 (homologue to nerfin-1 in mammals) is a useful marker for prognosis of neuroendocrine tumors. The dynamic expression of Nerfin-1 is regulated post-transcriptionally by multiple microRNAs; however, its post-translational regulation is still unclear. Here we showed that the protein turnover of Nerfin-1 is regulated by Slimb, the substrate adaptor of SCF Slimb ubiquitin ligase complex. Mechanistically, Slimb associates with Nerfin-1 and promotes it ubiquitination and degradation in Drosophila S2R + cells. Furthermore, we determined that the C-terminal half of Nerfin-1 (Nerfin-1 CT ) is required for its binding to Slimb. Genetic epistasis assays showed that Slimb misexpression antagonizes, while knock-down enhances the activity of Nerfin-1 CT in Drosophila eyes. Our data revealed a new link to understand the underlying mechanism for Nerfin-1 turnover in post-translational level, and provided useful insights in animal development and disease treatment by manipulating the activity of Slimb and Nerfin-1. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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