1. Activin A/Smads signaling pathway negatively regulates Oxygen Glucose Deprivation-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells.
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Xue, Long-Xing, Xu, Zhong-Hang, Wang, Jiao-Qi, Cui, Yang, Liu, Hong-Yu, Liang, Wen-Zhao, Ji, Qiu-Ye, He, Jin-Ting, Shao, Yan-Kun, Mang, Jing, and Xu, Zhong-Xin
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JAK-STAT pathway , *AUTOPHAGY , *NEURONAL ceroid-lipofuscinosis , *HYPERINSULINISM , *DEMYELINATION - Abstract
Activin A (Act A), a member of the transforming growth factor-beta (TGF-β), reduces neuronal apoptosis during cerebral ischemia through Act A/Smads signaling pathway. However, little is known about the effect of Act A/Smads pathway on autophagy in neurons. Here, we found that oxygen-glucose deprivation (OGD)-induced autophagy was suppressed by exogenous Act A in a concentration-dependent manner and enhanced by Act A/Smads pathway inhibitor (ActRIIA-Ab) in neuronal PC12 cells. These results indicate that Act A/Smads pathway negatively regulates autophagy in OGD-treated PC12 cells. In addition, we found that c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase pathways are involved in the OGD-induced autophagy. The activation of JNK and p38 MAPK pathways in OGD-treated PC12 cells was suppressed by exogenous Act A and enhanced by ActRIIA-Ab. Together, our results suggest that Act A/Smads signaling pathway negatively regulates OGD-induced autophagy via suppression of JNK and p38 MAPK pathways in neuronal PC12 cells. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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