1. First report of a novel plant lysozyme with both antifungal and antibacterial activities
- Author
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Wang, Shaoyun, Ng, Tzi Bun, Chen, Tianbao, Lin, Dongyun, Wu, Jinhong, Rao, Pingfan, and Ye, Xiuyun
- Subjects
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LYSOZYMES , *ANTIFUNGAL agents , *ANTIBACTERIAL agents , *CHROMATOGRAPHIC analysis - Abstract
Abstract: A novel lysozyme exhibiting antifungal activity and with a molecular mass of 14.4kDa in SDS–polyacrylamide gel electrophoresis was isolated from mung bean (Phaseolus mungo) seeds using a procedure that involved aqueous extraction, ammonium sulfate precipitation, ion exchange chromatography on CM-Sephadex, and high-performance liquid chromatography on POROS HS-20. Its N-terminal sequence was very different from that of hen egg white lysozyme. Its pI was estimated to be above 9.7. The specific activity of the lysozyme was 355U/mg at pH 5.5 and 30°C. The lysozyme exhibited a pH optimum at pH 5.5 and a temperature optimum at 55°C. It is reported herein, for the first time, that a novel plant lysozyme exerted an antifungal action toward Fusarium oxysporum, Fusarium solani, Pythium aphanidermatum, Sclerotium rolfsii, and Botrytis cinerea, in addition to an antibacterial action against Staphylococcus aureus. [Copyright &y& Elsevier]
- Published
- 2005
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