1. The miR-599 promotes non-small cell lung cancer cell invasion via SATB2.
- Author
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Tian, Wenjun, Wang, Guanghai, Liu, Yiqing, Huang, Zhenglan, Zhang, Caiqing, Ning, Kang, Yu, Cuixiang, Shen, Yajuan, Wang, Minghui, Li, Yuantang, Wang, Yong, Zhang, Bingchang, and Zhao, Yaoran
- Subjects
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CANCER treatment , *NON-small-cell lung carcinoma , *MICRORNA , *RNA interference , *RNA modification & restriction , *INHIBITION of cellular proliferation , *GENE expression - Abstract
MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we identified that miR-599 is up-regulated in non-small cell lung cancer (NSCLC) patients. It promoted NSCLC cell proliferation by negatively regulating SATB2. In NSCLC cell lines, CCK-8 proliferation assay indicated that the cell proliferation is promoted by miR-599 mimics. Transwell assay showed that miR-599 mimics promoted the invasion and migration of NSCLC cells. Luciferase assays confirmed that miR-599 directly binds to the 3'untranslated region of SATB2, and western blotting showed that miR-599 suppresses the expression of SATB2 at the protein level. This study indicates that miR-599 promotes proliferation and invasion of NSCLC cell lines via SATB2. The miR-599 may represent a potential therapeutic target for NSCLC treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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