Search

Urea plasma and Mycoplasma are parasitic organisms lack a cell wall and are thus extremely sensitive to external factors, including antibiotic. A selective agar was used to determine if the illness was present in the swab samples. Ten randomly selected samples from the colonies obtained were analyzed by PCR and analyzed phylogenetically using a phylogenetic tree. For this experiment, a specific PCR product was broken down using the pair of forward and reverse primers. After electrophoresis, the only band of amplified products was a single band of 1250, 712 and 785 base pairs in size. One hour and 30 minutes with a 1.5x TBE buffer are recommended. 100 DNA ladder and 1000 DNA ladder PCR can also detect Urea plasma and mycoplasma infections because of the low organism burden. Due to the development and deployment of molecular-based technologies during the past two decades, Mycoplasmas and Urea plasma may now be detected and identified in clinical specimens. The M. hominis and U. parvum species will require antibiotic susceptibility testing to ascertain their susceptibility. However, polyacrylamide gels that are run at higher voltages of 1.5x TBE using agarose can be utilized instead of agarose gels (siRNAs). This observation is critical due to the speed and ease of preparing agarose gel instead of polyacrylamide gel. [ABSTRACT FROM AUTHOR]