1. MOLECULAR DETECTION OF VIRULENCE GENES (PILB AND SID) IN PSEUDOMONAS SYRINGAE PV. LACHRYMANS ISOLATED FROM CUCUMBER LEAVES.
- Author
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Al-Fallooji, Saba A. K., Al-Hasnawi, Sabah L., and Al-Yasseen, Ahlam K.
- Subjects
MICROBIAL virulence ,PARASITISM ,PSEUDOMONAS syringae ,CUCUMBERS ,ELECTROPHORESIS - Abstract
Pseudomonas syringae pv. lachrymans possessed a lot of factors that play an important role in the development of parasitism and growth stages of the pathogen on plant host and consider a quantitative measure of plant disease. Therefore, this study was conducted to investigate some genes responsible for the virulence factors (pilB and Sid) in isolates of P. syringaepv. lachrymans that caused angular spot disease on cucumber leaves in different areas of Najaf province. The results showed that the pilB and Sid genes were amplified in fifty-two isolates of P. syringae pv. lachrymans, forty-five isolates containedpilB gene which responsible for adhesion factors through the emergence of binds in a molecular size of 586 base pairs on agarose gel after electrophoresis. Also forty-five isolates contained Sid gene, which responsible for the siderophore factor through the emergence of binds in a molecular size of 572 base pairs on agarose gel. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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