1. Bone morphogenetic protein (BMP)1-3 enhances bone repair.
- Author
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Grgurevic L, Macek B, Mercep M, Jelic M, Smoljanovic T, Erjavec I, Dumic-Cule I, Prgomet S, Durdevic D, Vnuk D, Lipar M, Stejskal M, Kufner V, Brkljacic J, Maticic D, and Vukicevic S
- Subjects
- Animals, Bone Morphogenetic Protein 1 genetics, Bone and Bones injuries, Cell Differentiation genetics, Cell Line, Collagen Type I metabolism, Fractures, Bone blood, Humans, Male, Mice, Osteoblasts cytology, Osteoblasts physiology, Osteogenesis genetics, Osteogenesis physiology, Rabbits, Rats, Rats, Sprague-Dawley, Recombinant Proteins genetics, Recombinant Proteins pharmacology, Wound Healing drug effects, Bone Morphogenetic Protein 1 pharmacology, Bone Morphogenetic Protein 1 physiology, Bone and Bones drug effects, Bone and Bones physiology, Fractures, Bone physiopathology, Wound Healing physiology
- Abstract
Members of the astacin family of metalloproteinases such as human bone morphogenetic protein 1 (BMP-1) regulate morphogenesis by processing precursors to mature functional extracellular matrix (ECM) proteins and several growth factors including TGFβ, BMP2, BMP4 and GFD8. We have recently discovered that BMP1-3 isoform of the Bmp-1 gene circulates in the human plasma and is significantly increased in patients with acute bone fracture. We hypothesized that circulating BMP1-3 might have an important role in bone repair and serve as a novel bone biomarker. When administered systemically to rats with a long bone fracture and locally to rabbits with a critical size defect of the ulna, recombinant human BMP1-3 enhanced bone healing. In contrast, neutralization of the endogenous BMP1-3 by a specific polyclonal antibody delayed the bone union. Invitro BMP1-3 increased the expression of collagen type I and osteocalcin in MC3T3-E(1) osteoblast like cells, and enhanced the formation of mineralized bone nodules from bone marrow mesenchymal stem cells. We suggest that BMP1-3 is a novel systemic regulator of bone repair., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
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