1. Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor
- Author
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Miguel Eduardo Carrion, Dragan Dimitrov, Peter Bohlen, Maureen Dougher-Vermazen, Denis Gospodarowicz, Bruce I. Terman, and Douglas C. Armellino
- Subjects
Molecular Sequence Data ,Biophysics ,Biochemistry ,Tropomyosin receptor kinase C ,Receptor tyrosine kinase ,chemistry.chemical_compound ,parasitic diseases ,Humans ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,biology ,Base Sequence ,Kinase insert domain receptor ,Cell Biology ,Protein-Tyrosine Kinases ,Molecular biology ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,Receptors, Vascular Endothelial Growth Factor ,chemistry ,Oligodeoxyribonucleotides ,Receptors, Mitogen ,ROR1 ,biology.protein ,Cancer research ,Tyrosine kinase ,Sequence Alignment ,Platelet-derived growth factor receptor - Abstract
Vascular endothelial cell growth factor (VEGF), also known as vascular permeability factor, is an endothelial cell mitogen which stimulates angiogenesis. Here we report that a previously identified receptor tyrosine kinase gene, KDR, endodes a receptor for VEGF. Expression of KDR in CMT-3 (cells which do not contain receptors for VEGF) allows for saturable 125I-VEGF binding with high affinity (KD = 75 pM). Affinity cross-linking of 125I-VEGF to KDR-transfected CMT-3 cells results in specific labeling of two proteins of Mr = 195 and 235 kDa. The KDR receptor tyrosine kinase shares structural similarities with a recently reported receptor for VEGF, f t , in a manner reminiscent of the similarities between the α and β forms of the PDGF receptors.
- Published
- 1992