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Your search keyword '"Fei-Fei Nie"' showing total 2 results
Start Over Author "Fei-Fei Nie" Journal biochemical and biophysical research communications
2 results on '"Fei-Fei Nie"'

1. Asparanin A induces G2/M cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells

Author

Wei Liu, Li Yang, Dandan Gong, Qinglong Guo, Qinsheng Dai, Qi Qi, Na Lu, Xue-Feng Huang, Ling-Yi Kong, and Fei-Fei Nie

Subjects
Cyclin-Dependent Kinase Inhibitor p21, G2 Phase, Carcinoma, Hepatocellular, Cell cycle checkpoint, Cell, Cyclin A, Biophysics, Apoptosis, Biochemistry, Cell Line, Tumor, CDC2 Protein Kinase, medicine, Humans, Cytotoxic T cell, Molecular Biology, Cyclin-dependent kinase 1, biology, Cell Cycle, Cyclin-Dependent Kinase 2, Liver Neoplasms, Cell Biology, Saponins, Cell cycle, Antineoplastic Agents, Phytogenic, Cell biology, medicine.anatomical_structure, Cell culture, Cancer research, biology.protein, Asparagus Plant, Cell Division
Abstract

We recently established that asparanin A, a steroidal saponin extracted from Asparagus officinalis L., is an active cytotoxic component. The molecular mechanisms by which asparanin A exerts its cytotoxic activity are currently unknown. In this study, we show that asparanin A induces G(2)/M phase arrest and apoptosis in human hepatocellular carcinoma HepG2 cells. Following treatment of HepG2 cells with asparanin A, cell cycle-related proteins such as cyclin A, Cdk1 and Cdk4 were down-regulated, while p21(WAF1/Cip1) and p-Cdk1 (Thr14/Tyr15) were up-regulated. Additionally, we observed poly (ADP-ribose) polymerase (PARP) cleavage and activation of caspase-3, caspase-8 and caspase-9. The expression ratio of Bax/Bcl-2 was increased in the treated cells, where Bax was also up-regulated. We also found that the expression of p53, a modulator of p21(WAF1/Cip1) and Bax, was not affected in asparanin A-treated cells. Collectively, our findings demonstrate that asparanin A induces cell cycle arrest and triggers apoptosis via a p53-independent manner in HepG2 cells. These data indicate that asparanin A shows promise as a preventive and/or therapeutic agent against human hepatoma.

Published
2009

2. LYG-202, a new flavonoid with a piperazine substitution, shows antitumor effects in vivo and in vitro

Author

Na Lu, Lei Tao, Fei-Fei Nie, Zhiyu Li, Wei Liu, Qi Qi, Qing Zhao, Shi Zeng, Jia Wang, Qinglong Guo, and Jing-Jing Rong

Subjects
Male, Cell Survival, Poly ADP ribose polymerase, Biophysics, Antineoplastic Agents, Apoptosis, Biochemistry, Piperazines, chemistry.chemical_compound, Mice, In vivo, Annexin, Cell Line, Tumor, Animals, Humans, DAPI, Molecular Biology, Piperazine, Caspase, Cell Proliferation, Flavonoids, Membrane Potential, Mitochondrial, Mice, Inbred ICR, biology, Cell Biology, Flavones, Molecular biology, Xenograft Model Antitumor Assays, In vitro, chemistry, Cell culture, biology.protein, Signal Transduction
Abstract

LYG-202 is a newly synthesized flavonoid with a piperazine substitution. We investigated the antitumor effect of LYG-202 in vivo and in vitro. We show that, LYG-202 significantly decreases tumor growth in mice inoculated with S180 sarcoma cells, compared with the control group. Meanwhile, the viabilities of various kinds of tumor cells were inhibited by LYG-202 with IC(50) values in the range of 4.80 to 27.70 microM. Then apoptosis induced by LYG-202 in HepG2 cells was characterized by DAPI staining and Annexin V/PI double staining and degradation of PARP was observed. Activation of the caspase cascade for both the extrinsic and intrinsic pathways was demonstrated, including caspase-8, -9, and -3. The results also showed that the expression of Bcl-2 protein decreased whereas that of Bax protein increased, leading to an increase of the Bax/Bcl-2 ratio. Our results demonstrated that LYG-202 exhibited strong antitumor effect in vivo and in vitro, involving with apoptosis induction.

Published
2009

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