1. CpG inhibits IgE class switch recombination through suppression of NFκB activity, but not through Id2 or Bcl6
- Author
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Akira Shimizu, Natsuki Nagata-Nakajima, Hiroyuki Gonda, Takashi Kusunoki, Yukiko Nambu, Mariko Kusunoki, Tomoya Katakai, Takeshi Tokuhisa, Manabu Sugai, Tatsutoshi Nakahata, Akemi Sakamoto, and Yoshifumi Yokota
- Subjects
Oligonucleotides ,Biophysics ,chemical and pharmacologic phenomena ,Biology ,Immunoglobulin E ,Biochemistry ,Mice ,chemistry.chemical_compound ,Transcription (biology) ,Proto-Oncogene Proteins ,Animals ,Molecular Biology ,Cells, Cultured ,Interleukin 4 ,Inhibitor of Differentiation Protein 2 ,B-Lymphocytes ,NF-kappa B ,Cell Biology ,BCL6 ,Molecular biology ,Immunoglobulin Switch Region ,DNA-Binding Proteins ,Repressor Proteins ,chemistry ,CpG site ,Immunoglobulin class switching ,Proto-Oncogene Proteins c-bcl-6 ,biology.protein ,CpG Islands ,Interleukin-4 ,DNA ,Signal Transduction ,Transcription Factors ,IRF4 - Abstract
The CpG motif in DNA plays a critical role in immunity via modulating the Th1/Th2 balance. In B cells, CpG-containing oligodeoxynucleotides (CpG ODNs) inhibit IL-4-mediated class switch recombination (CSR) to IgG1 and IgE through inhibition of the germline transcription (GLT) of these isotypes. However, the molecular mechanism of this inhibitory effect remains elusive. We showed here that Id2 and Bcl6, both of which inhibit IgE GLT and CSR, are not involved in this inhibitory pathway. We demonstrated that there is reduced activity of NF kappa B binding to the IgE promoter and a reduction of Irf4 protein in CpG ODN-treated B cells. These data indicate the critical role of NF kappa B and Irf4 in the regulation of IgE CSR through actions downstream of CpG signaling.
- Published
- 2005
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