1. Electronic cigarette aerosols and copper nanoparticles induce mitochondrial stress and promote DNA fragmentation in lung fibroblasts
- Author
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Irfan Rahman, Alison Elder, Pierrot Rutagarama, Isaac K. Sundar, Chad A. Lerner, and Tanveer Ahmad
- Subjects
0301 basic medicine ,Mitochondrial ROS ,Male ,Biophysics ,Metal Nanoparticles ,Nanotechnology ,Oxidative phosphorylation ,DNA Fragmentation ,Mitochondrion ,Electronic Nicotine Delivery Systems ,Biochemistry ,Article ,Cell Line ,Electron Transport Complex IV ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Interleukin 9 ,Fragmentation (cell biology) ,Molecular Biology ,chemistry.chemical_classification ,Aerosols ,Membrane Potential, Mitochondrial ,Reactive oxygen species ,Interleukin-6 ,Interleukin-9 ,Cell Biology ,respiratory system ,Cell biology ,Mitochondria ,030104 developmental biology ,chemistry ,Cell culture ,030220 oncology & carcinogenesis ,DNA fragmentation ,Reactive Oxygen Species ,Copper - Abstract
Oxidants or nanoparticles have recently been identified as constituents of aerosols released from various styles of electronic cigarettes (E-cigs). Cells in the lung may be directly exposed to these constituents and harbor reactive properties capable of incurring acute cell injury. Our results show mitochondria are sensitive to both E-cig aerosols and aerosol containing copper nanoparticles when exposed to human lung fibroblasts (HFL-1) using an Air-Liquid Interface culture system, evident by elevated levels of mitochondrial ROS (mtROS). Increased mtROS after aerosol exposure is associated with reduced stability of OxPhos electron transport chain (ETC) complex IV subunit and nuclear DNA fragmentation. Increased levels of IL-8 and IL-6 in HFL-1 conditioned media were also observed. These findings reveal both mitochondrial, genotoxic, and inflammatory stresses are features of direct cell exposure to E-cig aerosols which are ensued by inflammatory duress, raising a concern on deleterious effect of vaping. more...
- Published
- 2016