1. Inhibition of AMV DNA polymerase by polyriboadenylic acid containing epsilon-adenosine residues
- Author
-
Jack G. Chirikjian and Takis S. Papas
- Subjects
Adenosine ,Time Factors ,Transcription, Genetic ,Base pair ,DNA polymerase ,Polynucleotides ,Biophysics ,Oligonucleotides ,Acetaldehyde ,Tritium ,Biochemistry ,chemistry.chemical_compound ,medicine ,Chloroacetaldehyde ,Animals ,Thymine Nucleotides ,Molecular Biology ,Polymerase ,biology ,Avian Leukosis Virus ,Adenine Nucleotides ,RNA ,Substrate (chemistry) ,Cell Biology ,Molecular biology ,Kinetics ,Spectrometry, Fluorescence ,chemistry ,DNA Nucleotidyltransferases ,biology.protein ,Nucleic acid ,Chickens ,medicine.drug - Abstract
Summary Polyriboadenylic acid was treated with chloroacetaldehyde under conditions known to introduce e-adenosine groups. The degree of modification was monitored by increase in fluorescence intensity. Modified e-poly rA was found to be inhibitory when unprimed 70S AMV RNA was used as a substrate, suggesting direct competition with the poly rA tract of the RNA. Since e-poly rA cannot effectively base pair with nucleic acids normally involved in cellular processes, it has the potential of being useful as an inhibitor of oncogenic viral polymerases.
- Published
- 1974