1. Extensive modulation of a set of microRNAs in primary glioblastoma
- Author
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Manuela Ferracin, Chang Gong Liu, G. Sabatino, Maria Giulia Farace, Silvia Anna Ciafrè, Silvia Galardi, Annunziato Mangiola, Massimo Negrini, Carlo M. Croce, G. Maira, CIAFRE S.A., GALARDI S., MANGIOLA A., FERRACIN M., LIU C.G., SABATINO G., NEGRINI M., MAIRA G., CROCE C.M., and FARACE M.G.
- Subjects
Male ,Microarray ,Biophysics ,Biology ,Bioinformatics ,medicine.disease_cause ,Biochemistry ,Cell Line ,Reference Values ,Cell Line, Tumor ,microRNA ,medicine ,Humans ,Molecular Biology ,Gene ,Cancer ,Tumor-marker ,Tumor marker ,Gene Expression Regulation, Neoplastic ,Female ,Cell Differentiation ,MicroRNAs ,Brain ,Glioblastoma ,Neoplastic ,Tumor ,Cell lines ,Differentiation ,MicroRNA ,Oncogenes ,RNA ,Settore BIO/13 ,Translation (biology) ,Cell Biology ,medicine.disease ,Gene Expression Regulation ,Cancer research ,Carcinogenesis ,Human - Abstract
MicroRNAs (miRNAs) are short non-coding RNA molecules playing regulatory roles in animals and plants by repressing translation or cleaving RNA transcripts. The specific modulation of several microRNAs has been recently associated to some forms of human cancer, suggesting that these short molecules may represent a new class of genes involved in oncogenesis. In our study, we examined by microarray the global expression levels of 245 microRNAs in glioblastoma multiforme, the most frequent and malignant of primary brain tumors. The analysis of both glioblastoma tissues and glioblastoma cell lines allowed us to identify a group of microRNAs whose expression is significantly altered in this tumor. The most interesting results came from miR-221, strongly up-regulated in glioblastoma and from a set of brain-enriched miRNAs, miR-128, miR-181a, miR-181b, and miR-181c, which are down-regulated in glioblastoma.
- Published
- 2005