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Your search keyword '"Mao,Lijun"' showing total 4 results
Start Over Author "Mao,Lijun" Journal biochemical and biophysical research communications
4 results on '"Mao,Lijun"'

3. Smad3 mediates TGF-β1-induced collagen gel contraction by human lung fibroblasts

Author

Kobayashi, Tetsu, primary, Liu, Xiangde, additional, Wen, Fu-Qiang, additional, Kohyama, Tadashi, additional, Shen, Lei, additional, Wang, Xing Qi, additional, Hashimoto, Mitsuyoshi, additional, Mao, Lijun, additional, Togo, Shinsaku, additional, Kawasaki, Shin, additional, Sugiura, Hisatoshi, additional, Kamio, Koichiro, additional, and Rennard, Stephen I., additional

Published
2006
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4. Smad3 mediates TGF-beta1-induced collagen gel contraction by human lung fibroblasts.

Author

Kobayashi T, Liu X, Wen FQ, Kohyama T, Shen L, Wang XQ, Hashimoto M, Mao L, Togo S, Kawasaki S, Sugiura H, Kamio K, and Rennard SI

Subjects
Actins genetics, Actins metabolism, Actins ultrastructure, Animals, Biomechanical Phenomena, Cell Line, Fibroblasts metabolism, Gels, Humans, Lung metabolism, RNA, Small Interfering genetics, Rats, Smad2 Protein genetics, Smad2 Protein physiology, Smad3 Protein genetics, Transforming Growth Factor beta1, Collagen Type I physiology, Fibroblasts physiology, Lung cytology, Smad3 Protein physiology, Transforming Growth Factor beta physiology
Abstract

Transforming growth factor-beta1 (TGF-beta1) is a key mediator in tissue repair and fibrosis. Using small interference RNA (siRNA), the role of Smad2 and Smad3 in TGF-beta stimulation of human lung fibroblast contraction of collagenous matrix and induction of alpha-SMA and the role of alpha-SMA in contraction were assessed. HFL-1 cells were transfected with Smad2, Smad3 or control-siRNA, and cultured in floating Type I collagen gels +/- -TGF-beta1. TGF-beta1 augmented gel contraction in Smad2-siRNA- and control-siRNA-treated cells, but had no effect in Smad3-siRNA-treated cells. Similarly, TGF-beta1 upregulated alpha-SMA in Smad2-siRNA- and control-siRNA-treated cells, but had no effect on Smad3-siRNA-treated cells. Alpha-SMA-siRNA-treated cells did not contact the collagen gels with or without TGF-beta1, suggesting alpha-SMA is required for gel contraction. Thus, Smad3 mediates TGF-beta1-induced contraction and alpha-SMA induction in human lung fibroblasts. Smad3, therefore, could be a target for blocking contraction of human fibrotic tissue induced by TGF-beta1.

Published
2006
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