1. Chronic ethanol consumption inhibits repair of dimethylnitrosamine-induced DNA alkylation
- Author
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Anthony J. Garro, Mario Salvagnini, Siraj I. Mufti, and Charles S. Lieber
- Subjects
Male ,medicine.medical_specialty ,DNA Repair ,DNA repair ,Biophysics ,Methylation ,Biochemistry ,Dimethylnitrosamine ,Lesion ,chemistry.chemical_compound ,Reference Values ,Internal medicine ,medicine ,Animals ,Carbon Radioisotopes ,Molecular Biology ,Ethanol ,Rats, Inbred Strains ,DNA ,Cell Biology ,Carbohydrate ,Rats ,Alcoholism ,DNA Alkylation ,Endocrinology ,Liver ,chemistry ,Nitrosamine ,Nucleic acid ,medicine.symptom - Abstract
Chronic ethanol consumption causes a DNA repair deficiency. This was demonstrated in Sprague-Dawley rats injected with 14C-labeled dimethylnitrosamine after being pair-fed isocaloric, ethanol, or carbohydrate control diets for 4 weeks. Hepatic DNA was isolated from rats killed at intervals over a 36 hour period after administration of the nitrosamine and concentrations of alkylated guanine derivatives were measured. While N7-methylguanine was lost at equivalent rates from the DNA of both diet groups, 06methylguanine, a promutagenic lesion, persisted at higher levels for longer periods of time in the DNA from the alcohol-fed animals.
- Published
- 1988
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