1. Potential use of two aryl sulfotransferase cell-surface display systems to detoxify the endocrine disruptor bisphenol A.
- Author
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Nanudorn P, Thiengmag S, Whangsuk W, Mongkolsuk S, and Loprasert S
- Subjects
- Benzhydryl Compounds isolation & purification, Biotransformation, Endocrine Disruptors isolation & purification, Escherichia coli enzymology, Phenols isolation & purification, Water Pollutants, Chemical isolation & purification, Water Purification methods, Arylsulfotransferase metabolism, Benzhydryl Compounds metabolism, Desulfitobacterium enzymology, Endocrine Disruptors metabolism, Phenols metabolism, Water Pollutants, Chemical metabolism
- Abstract
Bisphenol A (BPA) is one of the most common toxic endocrine disruptors in the environment. A fast, efficient and environmental-friendly method for BPA detoxification is urgently needed. In this study, we show that the enzymatic transformation of BPA into a non-estrogenic BPA sulfate can be performed by the aryl sulfotransferase (ASTB) from Desulfitobacterium hafniense. We developed and compared two Escherichia coli ASTB cell-surface displaying systems using the outer membrane porin F (OprF) and the lipoprotein outer membrane A (Lpp-OmpA) as carriers. The surface localization of both fusion proteins was confirmed by Western blot and flow cytometry analysis as well as the enzymatic activity assay of the outer membrane fractions. Unfortunately, Lpp-OmpA-ASTB cells had an adverse effect on cell growth. In contrast, the OprF-ASTB cell biocatalyst was stable, expressing 70% of enzyme activity for 7 days. It also efficiently sulfated 90% of 5 mM BPA (1 mg/mL) in wastewater within 6 h., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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