1. Muscle regeneration by adipose tissue-derived adult stem cells attached to injectable PLGA spheres
- Author
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Sang Myun Cha, Sung Woo Cho, Seog Woon Kwon, Seung Hye Yang, Chan Wha Kim, Mi Jung Kim, Yu Suk Choi, Hea Nam Hong, Jhang Ho Pak, and Chan Jeoung Park
- Subjects
Muscle tissue ,Polymers ,Biophysics ,Adipose tissue ,Mice, Nude ,macromolecular substances ,Biochemistry ,chemistry.chemical_compound ,Mice ,Tissue engineering ,Polylactic Acid-Polyglycolic Acid Copolymer ,medicine ,Animals ,Humans ,Regeneration ,Lactic Acid ,Molecular Biology ,Microscopy, Confocal ,Tissue Engineering ,Chemistry ,Regeneration (biology) ,Muscles ,technology, industry, and agriculture ,Skeletal muscle ,Cell Differentiation ,Cell Biology ,Anatomy ,Cell biology ,PLGA ,medicine.anatomical_structure ,Adipose Tissue ,Immunostaining ,Polyglycolic Acid ,Adult stem cell ,Stem Cell Transplantation - Abstract
The [corrected] use of adult stem cells for cell-based tissue engineering and regeneration strategies represents a promising approach for skeletal muscle repair. We have evaluated the combination of adipose tissue-derived adult stem cells (ADSCs) obtained from autologous liposuction and injectable poly(lactic-co-glycolic acid) (PLGA) spheres for muscle regeneration. ADSCs attached to PLGA spheres and PLGA spheres alone were cultured in myogenic medium for 21 days and injected subcutaneously into the necks of nude mice. After 30 and 60 days, the mice were sacrificed, and newly formed tissues were analyzed by immunostaining, H and E staining, and RT-PCR. We found that ADSCs attached to PLGA spheres, but not PLGA spheres alone, were able to generate muscle tissue. These findings suggest that ADSCs and PLGA spheres are useful materials for muscle tissue engineering and that their combination can be used in clinical settings for muscle regeneration.
- Published
- 2006