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Start Over Author "T Saishoji" Journal biochemical and biophysical research communications
2 results on '"T Saishoji"'

1. Advanced glycation end products stimulate plasminogen activator activity via GM-CSF in RAW 264.7 cells

Author

Yoshiteru Jinnouchi, T. Saishoji, Kazuyoshi Ikeda, Hiroyuki Sano, Minetaro Ogawa, Takayuki Higashi, and Seikoh Horiuchi

Subjects
Glycosylation, Endocytic cycle, Molecular Sequence Data, Biophysics, Serum albumin, Plasminogen activator activity, Biochemistry, Cell Line, Mice, Plasminogen Activators, Glycation, Plasminogen Activator Inhibitor 1, Animals, Humans, RNA, Messenger, Molecular Biology, RAW 264.7 Cells, DNA Primers, Extracellular Matrix Proteins, biology, Base Sequence, Chemistry, Macrophages, Granulocyte-Macrophage Colony-Stimulating Factor, Cell Biology, Urokinase-Type Plasminogen Activator, In vitro, Cell biology, Tissue Plasminogen Activator, biology.protein, Antibody, Plasminogen activator
Abstract

The effects of advanced glycation end products (AGE) on the plasminogen activator (PA) activity were investigated with murine macrophage cell line RAW 264.7 cells. AGE-bovine serum albumin (BSA) showed a dose-dependent induction for the urokinase-type PA (uPA) activity. The uPA induction by AGE-BSA was effectively suppressed by the antibody against Fl granulocyte-macrophage colony-stimulating factor (GM-CSF). The uPA activity of these cells was also induced by ligands for the macrophage scavenger receptor (MSR). These data provide evidence that AGE-BSA. stimulates the uPA activity via GM-CSF through MSR in RAW cells. These findings, taken together with a recent demonstration of endocytic uptake of AGE-proteins by MSR in vitro and the presence of AGE-proteins in atherosclerotic lesions, strongly suggest that the uPA induction by AGE-proteins via MSR plays an important role in human atherogenesis.

Published
1995

2. Advanced glycation end products stimulate plasminogen activator activity via GM-CSF in RAW 264.7 cells.

Author

Saishoji T, Higashi T, Ikeda K, Sano H, Jinnouchi Y, Ogawa M, and Horiuchi S

Subjects
Animals, Base Sequence, Cell Line, DNA Primers genetics, Extracellular Matrix Proteins chemistry, Extracellular Matrix Proteins metabolism, Glycosylation, Humans, Macrophages metabolism, Mice, Molecular Sequence Data, Plasminogen Activator Inhibitor 1 biosynthesis, Plasminogen Activator Inhibitor 1 genetics, Plasminogen Activators genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Tissue Plasminogen Activator genetics, Tissue Plasminogen Activator metabolism, Urokinase-Type Plasminogen Activator genetics, Urokinase-Type Plasminogen Activator metabolism, Granulocyte-Macrophage Colony-Stimulating Factor metabolism, Plasminogen Activators metabolism
Abstract

The effects of advanced glycation end products (AGE) on the plasminogen activator (PA) activity were investigated with murine macrophage cell line RAW 264.7 cells. AGE-bovine serum albumin (BSA) showed a dose-dependent induction for the urokinase-type PA (uPA) activity. The uPA induction by AGE-BSA was effectively suppressed by the antibody against granulocyte-macrophage colony-stimulating factor (GM-CSF). The uPA activity of these cells was also induced by ligands for the macrophage scavenger receptor (MSR). These data provide evidence that AGE-BSA stimulates the uPA activity via GM-CSF through MSR in RAW cells. These findings, taken together with a recent demonstration of endocytic uptake of AGE-proteins by MSR in vitro and the presence of AGE-proteins in atherosclerotic lesions, strongly suggest that the uPA induction by AGE-proteins via MSR plays an important role in human atherogenesis.

Published
1995
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